Semliki Forest Virus (SFV) is an enveloped alphavirus that infects cells by a low-pH-dependent membrane residues 79 and 97. Prior mutagenesis studies demonstrated that an E1 G91d mutation blocks both virus-membrane fusion and the formation of highly stable E1 trimer believed to be a critical fusion intermediate. We have here demonstrated that the G91d mutant was also inactive in hemifusion, suggesting that the E1 homotrimer is important in the initial stages of lipid mixing. Reverent analysis of a G91 deletion mutant indicated that G91 was crucial for the viability of SFV. In contrast, G83D mutation produced infectious virus with both efficient fusion and homotrimer formation. thus, the G83 position, although highly conserved among alphaviruses was functional if replaced with a charged amino acid.
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Yeshiva Univ Albert Einstein Coll Med, Dept Cell Biol, Bronx, NY 10461 USAYeshiva Univ Albert Einstein Coll Med, Dept Cell Biol, Bronx, NY 10461 USA
Ahn, A
Klimjack, MR
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Yeshiva Univ Albert Einstein Coll Med, Dept Cell Biol, Bronx, NY 10461 USAYeshiva Univ Albert Einstein Coll Med, Dept Cell Biol, Bronx, NY 10461 USA
Klimjack, MR
Chatterjee, PK
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Yeshiva Univ Albert Einstein Coll Med, Dept Cell Biol, Bronx, NY 10461 USAYeshiva Univ Albert Einstein Coll Med, Dept Cell Biol, Bronx, NY 10461 USA
Chatterjee, PK
Kielian, M
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Yeshiva Univ Albert Einstein Coll Med, Dept Cell Biol, Bronx, NY 10461 USAYeshiva Univ Albert Einstein Coll Med, Dept Cell Biol, Bronx, NY 10461 USA
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Yeshiva Univ Albert Einstein Coll Med, Dept Cell Biol, Bronx, NY 10461 USAYeshiva Univ Albert Einstein Coll Med, Dept Cell Biol, Bronx, NY 10461 USA
Chatterjee, PK
Vashishtha, M
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Yeshiva Univ Albert Einstein Coll Med, Dept Cell Biol, Bronx, NY 10461 USAYeshiva Univ Albert Einstein Coll Med, Dept Cell Biol, Bronx, NY 10461 USA
Vashishtha, M
Kielian, M
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Yeshiva Univ Albert Einstein Coll Med, Dept Cell Biol, Bronx, NY 10461 USAYeshiva Univ Albert Einstein Coll Med, Dept Cell Biol, Bronx, NY 10461 USA
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Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Piscataway, NJ 08854 USAUniv Med & Dent New Jersey, Robert Wood Johnson Med Sch, Piscataway, NJ 08854 USA
Cleverley, DZ
Lenard, J
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Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Piscataway, NJ 08854 USAUniv Med & Dent New Jersey, Robert Wood Johnson Med Sch, Piscataway, NJ 08854 USA
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Yeshiva Univ Albert Einstein Coll Med, Dept Cell Biol, Bronx, NY 10461 USAYeshiva Univ Albert Einstein Coll Med, Dept Cell Biol, Bronx, NY 10461 USA
Ahn, A
Klimjack, MR
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Yeshiva Univ Albert Einstein Coll Med, Dept Cell Biol, Bronx, NY 10461 USAYeshiva Univ Albert Einstein Coll Med, Dept Cell Biol, Bronx, NY 10461 USA
Klimjack, MR
Chatterjee, PK
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Yeshiva Univ Albert Einstein Coll Med, Dept Cell Biol, Bronx, NY 10461 USAYeshiva Univ Albert Einstein Coll Med, Dept Cell Biol, Bronx, NY 10461 USA
Chatterjee, PK
Kielian, M
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Yeshiva Univ Albert Einstein Coll Med, Dept Cell Biol, Bronx, NY 10461 USAYeshiva Univ Albert Einstein Coll Med, Dept Cell Biol, Bronx, NY 10461 USA
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Yeshiva Univ Albert Einstein Coll Med, Dept Cell Biol, Bronx, NY 10461 USAYeshiva Univ Albert Einstein Coll Med, Dept Cell Biol, Bronx, NY 10461 USA
Chatterjee, PK
Vashishtha, M
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Yeshiva Univ Albert Einstein Coll Med, Dept Cell Biol, Bronx, NY 10461 USAYeshiva Univ Albert Einstein Coll Med, Dept Cell Biol, Bronx, NY 10461 USA
Vashishtha, M
Kielian, M
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h-index: 0
机构:
Yeshiva Univ Albert Einstein Coll Med, Dept Cell Biol, Bronx, NY 10461 USAYeshiva Univ Albert Einstein Coll Med, Dept Cell Biol, Bronx, NY 10461 USA
机构:
Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Piscataway, NJ 08854 USAUniv Med & Dent New Jersey, Robert Wood Johnson Med Sch, Piscataway, NJ 08854 USA
Cleverley, DZ
Lenard, J
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h-index: 0
机构:
Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Piscataway, NJ 08854 USAUniv Med & Dent New Jersey, Robert Wood Johnson Med Sch, Piscataway, NJ 08854 USA