Real-time two-photon confocal microscopy using a femtosecond, amplified Ti:sapphire system

被引：97

作者：

Brakenhoff, GJ

Squier, J

Norris, T

Bliton, AC

Wade, MH

Athey, B

机构：

[1] UNIV MICHIGAN, CTR ULTRAFAST OPT SCI, ANN ARBOR, MI 48109 USA

[2] MERIDIAN INSTRUMENTS INC, OKEMOS, MI 48864 USA

[3] UNIV MICHIGAN, SCH MED, DEPT ANAT & CELL BIOL, ANN ARBOR, MI 48109 USA

来源：

JOURNAL OF MICROSCOPY | 1996年 / 181卷

关键词：

bilateral; confocal; real-time imaging; sectioning; three-dimensional imaging; TPA; two-photon fluorescence;

D O I：

10.1046/j.1365-2818.1996.97379.x

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

The bilateral imaging approach known from confocal applications operating in the line mode was used to realize real-time two-photon imaging. It is shown that the sectioning inherent to two-photon imaging could be improved by the introduction of a confocal lie aperture in the imaging path. Using a high-power, low-repetition-rate amplified Ti:sapphire system, various biological objects were visualized including live boar sperm.

引用

页码：253 / 259

页数：7

共 16 条

[1]

AGARD DA, 1989, METHOD CELL BIOL, V30, P353

[2] 3-DIMENSIONAL CHROMATIN DISTRIBUTION IN NEURO-BLASTOMA NUCLEI SHOWN BY CONFOCAL SCANNING LASER MICROSCOPY [J].