Reversible Reaction-Based Fluorescent Probe for Real-Time Imaging of Glutathione Dynamics in Mitochondria

被引：113

作者：

Chen, Jianwei ^{[1
]}

Jiang, Xiqian ^{[1
]}

Zhang, Chengwei ^{[1
]}

MacKenzie, Kevin R. ^{[1
,2
]}

Stossi, Fabio ^{[3
,4
]}

Palzkill, Timothy ^{[1
,2
]}

Wang, Meng C. ^{[5
,6
]}

Wang, Jin ^{[1
,2
,3
]}

机构：

[1] Baylor Coll Med, Dept Pharmacol & Chem Biol, Houston, TX 77030 USA

[2] Baylor Coll Med, Ctr Drug Discovery, Houston, TX 77030 USA

[3] Baylor Coll Med, Dept Mol & Cellular Biol, Houston, TX 77030 USA

[4] Baylor Coll Med, Integrated Microscopy Core, Adv Technol Cores, Houston, TX 77030 USA

[5] Baylor Coll Med, Dept Mol & Human Genet, Houston, TX 77030 USA

[6] Baylor Coll Med, Huffington Ctr Aging, Houston, TX 77030 USA

来源：

ACS SENSORS | 2017年 / 2卷 / 09期

基金：

美国国家卫生研究院;

关键词：

glutathione; mitochondria; oxidative stress; sensors and probes; fluorescence imaging; flow cytometry;

D O I：

10.1021/acssensors.7b00425

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report a mitochondria-specific glutathione (GSH) probe designated as Mito-RealThiol (MitoRT) that can monitor in vivo real-time mitochondria] glutathione dynamics, and apply this probe to follow mitochondrial GSH dynamic changes in living cells for the first time. MitoRT can be utilized in confocal microscopy, super-resolution fluorescence imaging, and flow cytometry systems. Using MitoRT, we demonstrate that cells have a high priority to maintain the GSH level in mitochondria compared to the cytosol not only under normal growing conditions but also upon oxidative stress.

引用

页码：1257 / 1261

页数：5

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