Safflower polysaccharide induces cervical cancer cell apoptosis via inhibition of the PI3K/Akt pathway

被引：15

作者：

Yang, J. ^{[1
]}

Wang, R. ^{[1
]}

Feng, Q. ^{[1
]}

Wang, Y. -X. ^{[1
]}

Zhang, Y. -Y. ^{[1
]}

Wu, W. -H. ^{[1
]}

Ge, P. -L. ^{[1
]}

Qi, J. -P. ^{[2
]}

机构：

[1] Heilongjiang Univ Chinese Med, Harbin 150040, Heilongjiang, Peoples R China

[2] Harbin Med Univ, Affiliated Hosp 1, Harbin 150001, Heilongjiang, Peoples R China

来源：

SOUTH AFRICAN JOURNAL OF BOTANY | 2018年 / 118卷

基金：

中国国家自然科学基金;

关键词：

Safflower polysaccharide; Cervical cancer; Apoptosis; ACTIVATION; PROTEINS; DOMAIN; TARGET; DEATH; AKT; BAD;

D O I：

10.1016/j.sajb.2018.07.028

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Safflower polysaccharide (SPS) is a major active component of Carthamus tinctorius L. Previous reports have demonstrated that SPS shows cytotoxic activity against various types of cancer cells; however, its anti-cervical cancer effect remains unclear. Therefore, we explored the effect of SPS treatment of a cervical cancer cell line (HeLa). SPS significantly inhibited cell proliferation and increased the rate of cell apoptosis as determined by TdT-mediated dUTP nick-end labeling assay, Hoechst 33342 staining, and double staining with annexin V-fluorescein isothiocyante/propidium iodide. Furthermore, western blot and real-time polymerase chain reaction revealed that SPS down-regulated mammalian target of rapamycin and protein kinase B and up-regulated Bcl-2-associated death promoter. These results suggest that SPS-induced apoptosis may occur through down-regulation of the phosphatidylinositol-3-kinase/AKT pathway. Collectively, these findings implicate SPS as a candidate for the development of anti-cervical cancer drugs. (c) 2018 SAAB. Published by Elsevier B.V. All rights reserved.

引用

页码：209 / 215

页数：7

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