A considerable share of bacterial species maintains multipartite genomes. Precise coordination of genome replication and segregation with cell growth and division is vital for proliferation of these bacteria. The a-proteobacterium Sinorhizobium meliloti possesses a tripartite genome composed of one chromosome and the megaplasmids pSymA and pSymB. Here, we investigated the spatiotemporal pattern of segregation of these S. meliloti replicons at single cell level. Duplication of chromosomal and megaplasmid origins of replication occurred spatially and temporally separated, and only once per cell cycle. Tracking of FROS (fluorescent repressor operator system)-labelled origins revealed a strict temporal order of segregation events commencing with the chromosome followed by pSymA and then by pSymB. The repA2B2C2 region derived from pSymA was sufficient to confer the spatiotemporal behaviour of this megaplasmid to a small plasmid. Altering activity of the ubiquitous prokaryotic replication initiator DnaA, either positively or negatively, resulted in an increase in replication initiation events or G1 arrest of the chromosome only. This suggests that interference with DnaA activity does not affect replication initiation control of the megaplasmids.
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Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Programa Genom Evolut, Cuernavaca 62191, Morelos, MexicoUniv Nacl Autonoma Mexico, Ctr Ciencias Genom, Programa Genom Evolut, Cuernavaca 62191, Morelos, Mexico
Cervantes-Rivera, Ramon
Pedraza-Lopez, Francisco
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Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Programa Genom Evolut, Cuernavaca 62191, Morelos, MexicoUniv Nacl Autonoma Mexico, Ctr Ciencias Genom, Programa Genom Evolut, Cuernavaca 62191, Morelos, Mexico
Pedraza-Lopez, Francisco
Perez-Segura, Gabriela
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Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Programa Genom Evolut, Cuernavaca 62191, Morelos, MexicoUniv Nacl Autonoma Mexico, Ctr Ciencias Genom, Programa Genom Evolut, Cuernavaca 62191, Morelos, Mexico
Perez-Segura, Gabriela
Cevallos, Miguel A.
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Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Programa Genom Evolut, Cuernavaca 62191, Morelos, MexicoUniv Nacl Autonoma Mexico, Ctr Ciencias Genom, Programa Genom Evolut, Cuernavaca 62191, Morelos, Mexico
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Univ Namur UNamur, Microorganisms Biol Res Unit URBM, Namur, BelgiumUniv Namur UNamur, Microorganisms Biol Res Unit URBM, Namur, Belgium
Deghelt, Michael
Mullier, Caroline
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Univ Namur UNamur, Microorganisms Biol Res Unit URBM, Namur, BelgiumUniv Namur UNamur, Microorganisms Biol Res Unit URBM, Namur, Belgium
Mullier, Caroline
Sternon, Jean-Francois
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Univ Namur UNamur, Microorganisms Biol Res Unit URBM, Namur, BelgiumUniv Namur UNamur, Microorganisms Biol Res Unit URBM, Namur, Belgium
Sternon, Jean-Francois
Francis, Nayla
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Univ Namur UNamur, Microorganisms Biol Res Unit URBM, Namur, BelgiumUniv Namur UNamur, Microorganisms Biol Res Unit URBM, Namur, Belgium
Francis, Nayla
Laloux, Geraldine
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Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Yale Univ, Howard Hughes Med Inst, New Haven, CT 06520 USA
Yale Univ, Sch Med, Dept Microbial Pathogenesis, New Haven, CT 06520 USAUniv Namur UNamur, Microorganisms Biol Res Unit URBM, Namur, Belgium
Laloux, Geraldine
Dotreppe, Delphine
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Univ Namur UNamur, Microorganisms Biol Res Unit URBM, Namur, BelgiumUniv Namur UNamur, Microorganisms Biol Res Unit URBM, Namur, Belgium
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Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Programa Genom Evolut, Cuernavaca 62191, Morelos, MexicoUniv Nacl Autonoma Mexico, Ctr Ciencias Genom, Programa Genom Evolut, Cuernavaca 62191, Morelos, Mexico
Cervantes-Rivera, Ramon
Pedraza-Lopez, Francisco
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h-index: 0
机构:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Programa Genom Evolut, Cuernavaca 62191, Morelos, MexicoUniv Nacl Autonoma Mexico, Ctr Ciencias Genom, Programa Genom Evolut, Cuernavaca 62191, Morelos, Mexico
Pedraza-Lopez, Francisco
Perez-Segura, Gabriela
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h-index: 0
机构:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Programa Genom Evolut, Cuernavaca 62191, Morelos, MexicoUniv Nacl Autonoma Mexico, Ctr Ciencias Genom, Programa Genom Evolut, Cuernavaca 62191, Morelos, Mexico
Perez-Segura, Gabriela
Cevallos, Miguel A.
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Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Programa Genom Evolut, Cuernavaca 62191, Morelos, MexicoUniv Nacl Autonoma Mexico, Ctr Ciencias Genom, Programa Genom Evolut, Cuernavaca 62191, Morelos, Mexico
机构:
Univ Namur UNamur, Microorganisms Biol Res Unit URBM, Namur, BelgiumUniv Namur UNamur, Microorganisms Biol Res Unit URBM, Namur, Belgium
Deghelt, Michael
Mullier, Caroline
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h-index: 0
机构:
Univ Namur UNamur, Microorganisms Biol Res Unit URBM, Namur, BelgiumUniv Namur UNamur, Microorganisms Biol Res Unit URBM, Namur, Belgium
Mullier, Caroline
Sternon, Jean-Francois
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h-index: 0
机构:
Univ Namur UNamur, Microorganisms Biol Res Unit URBM, Namur, BelgiumUniv Namur UNamur, Microorganisms Biol Res Unit URBM, Namur, Belgium
Sternon, Jean-Francois
Francis, Nayla
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h-index: 0
机构:
Univ Namur UNamur, Microorganisms Biol Res Unit URBM, Namur, BelgiumUniv Namur UNamur, Microorganisms Biol Res Unit URBM, Namur, Belgium
Francis, Nayla
Laloux, Geraldine
论文数: 0引用数: 0
h-index: 0
机构:
Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Yale Univ, Howard Hughes Med Inst, New Haven, CT 06520 USA
Yale Univ, Sch Med, Dept Microbial Pathogenesis, New Haven, CT 06520 USAUniv Namur UNamur, Microorganisms Biol Res Unit URBM, Namur, Belgium
Laloux, Geraldine
Dotreppe, Delphine
论文数: 0引用数: 0
h-index: 0
机构:
Univ Namur UNamur, Microorganisms Biol Res Unit URBM, Namur, BelgiumUniv Namur UNamur, Microorganisms Biol Res Unit URBM, Namur, Belgium