NMR analysis of budding yeast metabolomics: a rapid method for sample preparation

被引：20
作者：
Airoldi, C. [1 ,2 ]
Tripodi, F. [1 ,2 ]
Guzzi, C. [1 ,2 ]
Nicastro, R. [1 ,2 ]
Coccetti, P. [1 ,2 ]
机构：
[1] Univ Milano Bicocca, Dept Biotechnol & Biosci, Milan, Italy
[2] SYSBIO, Ctr Syst Biol, I-20126 Milan, Italy
来源：
MOLECULAR BIOSYSTEMS | 2015年 / 11卷 / 02期
关键词：
GLYCEROL-3-PHOSPHATE DEHYDROGENASE MUTANTS; GLYCEROL 3-PHOSPHATE DEHYDROGENASE; GLOBAL METABOLITE ANALYSIS; SACCHAROMYCES-CEREVISIAE; EXTRACTION; SPECTROSCOPY; ETHANOL; STRESS; MODEL;
D O I：
10.1039/c4mb00452c
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Here we propose the optimization of a rapid and reproducible protocol for intracellular metabolite extraction from yeast cells and their metabolic profiling by H-1-NMR spectroscopy. The protocol reliability has been validated through comparison between the metabolome of cells in different phases of growth or with different genetic backgrounds.
引用
收藏
页码：379 / 383
页数：5
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