Protocol Making Droplet-Embedded Vesicles to Model Cellular Lipid Droplets

被引：13

作者：

Chorlay, Aymeric ^{[1
]}

Santinho, Alexandre ^{[1
]}

Thiam, Abdou Rachid ^{[1
]}

机构：

[1] Univ Paris, Univ PSL, Sorbonne Univ, CNRS,Lab Phys Ecole Normale Super,ENS, F-75005 Paris, France

来源：

STAR PROTOCOLS | 2020年 / 1卷 / 03期

关键词：

D O I：

10.1016/j.xpro.2020.100116

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a reproducible protocol to prepare droplet-embedded vesicles (DEVs) consisting of an oil droplet embedded within a phospholipid bilayer. This model system mimics a cellular lipid droplet (LD) in physical contact with the endoplasmic reticulum (ER) bilayer. It has the advantage that the lipid compo-sition and the biophysical properties of the droplet and the bilayer are controlled and tunable. DEVs can be used to study LD biogenesis factors and determinants of protein binding between ER and LD interfaces.For complete details on the use and execution of this protocol, please refer to Chorlay and Thiam (2020) and Santinho et al. (2020).

引用

页数：11

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