A novel chimeric prophage vB_LdeS-phiJB from commercial Lactobacillus delbrueckii subsp bulgaricus

被引：8

作者：

Guo, Tingting ^{[1
]}

Zhang, Chenchen ^{[1
]}

Xin, Yongping ^{[1
]}

Xin, Min ^{[1
]}

Kong, Jian ^{[1
]}

机构：

[1] Shandong Univ, State Key Lab Microbial Technol, 27 Shanda Nanlu, Jinan 250100, Peoples R China

来源：

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY | 2016年 / 43卷 / 05期

基金：

中国博士后科学基金; 中国国家自然科学基金;

关键词：

Lactobacillus delbrueckii subsp bulgaricus; Comparative genomic; Chimeric phage; FERMENTUM TEMPERATE BACTERIOPHAGE; PHAGE-RESISTANT MUTANTS; LL-H; LIPOTEICHOIC ACIDS; GENETIC ELEMENTS; GENOME SEQUENCE; LACTIS; ADSORPTION; LYSOGENY; STRAINS;

D O I：

10.1007/s10295-016-1739-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Prophage vB_LdeS-phiJB (phiJB) was induced by mitomycin C and UV radiation from the Lactobacillus delbrueckii subsp. bulgaricus SDMCC050201 isolated from a Chinese yoghurt sample. It has an isometric head and a non-contractile tail with 36,969 bp linear double-stranded DNA genome, which is classified into the group a of Lb. delbrueckii phages. The genome of phiJB is highly modular with functionally related genes clustered together. Unexpectedly, there is no similarity of its DNA replication module to any phages that have been reported, while it consists of open-reading frames homologous to the proteins of Lactobacillus strains. Comparative genomic analysis indicated that its late gene clusters, integration/lysogeny modules and DNA replication module derived from different evolutionary ancestors and integrated into a chimera. Our results revealed a novel chimeric phage of commercial Lb. delbrueckii and will broaden the knowledge of phage diversity in the dairy industry.

引用

页码：681 / 689

页数：9

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