Enhanced pilot bioremediation of oily sludge from petroleum refinery disposal under hot-summer Mediterranean climate

被引:13
作者
Ben Said, Olfa [1 ,2 ]
Cravo-Laureau, Cristiana [2 ]
Armougom, Fabrice [3 ]
Cipullo, Sabrina [4 ]
Ben Khelil, Meriem [1 ]
Yahiya, Marouen Ben Haj [4 ]
Douihech, Abdeljabar [5 ]
Beyrem, Hamouda [1 ]
Coulon, Frederic [4 ]
Duran, Robert [2 ]
机构
[1] Bizerte Fac Sci, Environm Biomonitoring Lab LBE LR ES 14 01, Zarzouna 7021, Bizerte, Tunisia
[2] Univ Pau & Pays Adour, MELODY Grp, Equipe Environm & Microbiol, UPPA E2S,IPREM UMR CNRS 5254, BP 1155, F-64013 Pau, France
[3] Mediterranean Inst Oceanog, MEB Team Bat OCEANOMED, F-13288 Marseille 9, France
[4] Cranfield Univ, Sch Water Energy & Environm, Cranfield MK43 0AL, Beds, England
[5] Ctr Tech Chim CTC Minist Ind Tunis, Tunis, Tunisia
关键词
Bacterial consortium; Illumina sequencing; Metal; Hydrocarbons; Ecotoxicity; Ecological succession; POLYCYCLIC AROMATIC-HYDROCARBONS; SULFATE-REDUCING BACTERIA; SP NOV; EMENDED DESCRIPTIONS; MICROBIAL ACTIVITY; DIVERSITY; SEDIMENTS; DEGRADATION; COMMUNITIES; STRAINS;
D O I
10.1016/j.eti.2021.102037
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Large pilot scale bioremediation approaches were implemented for the treatments of oily sludge (OS) characterised by alkaline pH (pH > 9), high concentration of metals (3% dry weight) and high total petroleum hydrocarbons content (TPH) ranging between 22,000 and 67,300 mg kg(-1) from a Tunisian petroleum refinery. The treatments included bioaugmentation and biostimulation approaches with autochthonous isolated bacterial strains and consortia. Chemical, microbial, and ecotoxicological analyses were performed over a period of 180 days incubation. The bioremediation treatments favoured the development of Proteobacteria, Firmicutes and Bacteroidetes following an ecological succession of specialist bacterial groups, first associated to hydrocarbon degradation (e.g. Marinobacter and Alcanivorax) that resulted in a greater extent of TPH-degradation (up to 80%), and the selection of metal resistant bacteria including Hyphomonas, Phaeobacter, and Desulfuromusa. The best performances were obtained when bioaugmentation and biostimulation were combined. Over 90% of the TPH initial concentration was degraded over 180 days, which was accompanied with a 3-fold reduction of ecotoxicity. Our study demonstrates the efficacy of large pilot scale bioremediation of highly contaminated oily sludge, providing the evidence that the management of autochthonous microbial communities is of paramount importance for the success of the bioremediation process. (C) 2021 The Authors. Published by Elsevier B.V.
引用
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页数:15
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