Structure and gene cluster of the O-antigen of Enterobacter cloacae G2559

被引：1
作者：
Wang, Min [1 ,2 ]
Filatov, Andrei V. [3 ]
Shashkov, Alexander S. [3 ]
Liu, Jiafei [4 ]
V. Perepelov, Andrei [3 ]
机构：
[1] Minist Educ, Key Lab Mol Microbiol & Technol, Tianjin, Peoples R China
[2] Nankai Univ, TEDA Inst Biol Sci & Biotechnol, TEDA, Tianjin, Peoples R China
[3] Russian Acad Sci, ND Zelinsky Inst Organ Chem, Moscow 119991, Russia
[4] Nankai Univ, Tianjin Union Med Ctr, Dept Colorectal Surg, Tianjin, Peoples R China
来源：
CARBOHYDRATE RESEARCH | 2022年 / 519卷
基金：
俄罗斯基础研究基金会;
关键词：
Enterobactercloacae; Bacterialpolysaccharidestructure; O-antigen; Lipopolysaccharide; O-antigengenecluster;
D O I：
10.1016/j.carres.2022.108612
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The O-polysaccharide (OPS) was isolated by mild acid degradation of the lipopolysaccharide of Enterobacter cloacae G2559 and studied by sugar analysis along with 1D and 2D H-1 and C-13 NMR spectroscopy. The following structure of the branched pentasaccharide repeating unit was established. alpha-D-Glep1 down arrow 4 -> 6)-alpha-D-Manp-(1 -> 2)-alpha-D-Manp-(1 ->)-beta-D-Manp-(1 -> 3)-alpha-D-FuepNAc-(1 -> The O-antigen gene cluster of Enterobacter cloacae G2559 was sequenced. The gene functions were tentatively assigned by comparison with sequences in the available databases and found to be in full agreement with the O -antigen structure.
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页数：4
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