This study evaluated the working postures, working conditions and musculoskeletal outcomes among different agricultural workers in Iran. Data were collected using questionnaires and direct observations of posture (using the Rapid Upper Limb Assessment [RULA] method) from 377 farmers including 150 rice farmers, 122 vegetable growers, and 105 greenhouse workers. The overall prevalence of musculoskeletal symptoms, particularly in the low back (75.1%), knees (62.1%), upper back (61.55%), and neck (59.9%) was very high, and 72.6% of farmers experienced more than three locations of pain/discomfort. The mean RULA grand score of 6.7 highlighted that most farmers needed immediate investigation and changes in their working postures. Being female (neck pain), occupation (rice farmers-low back pain), number of years worked as an agricultural worker (neck and knee pain), prolonged daily working hours (upper back pain), job satisfaction (neck and low back pain), fast working (upper back pain), and working postures (neck, upper back, low back and knee pain) were independently associated with the presence of musculoskeletal symptoms. These findings emphasise the need for multiple component interventions to improve the working conditions of this population.