PRODUCTION OF LIPASES FROM ZYGOSACCHAROMYCES MRAKII TS16

There are number of applications of yeast lipases, hence new and novel strains are constantly explored for higher yields of lipases and improved features. In this work, effect of parameters on the production of lipases from Zygosaccharomyces mrakii TS16 was studied using statistical tool, Plackett-Burman design (PBD). Initially, PBD was generated to study the effect of temperature, pH, incubation period, concentration of olive oil and media type on lipase production. Analysis of the data showed that only one of the factors, media type, had significant effect on lipases production. Consequently, another PBD was generated to evaluate the effect of sources of nitrogen, sugar, lipid along with supplementation of detergent and recycled cooking oil on lipases production by TS16. Although, none of the factors was found having significant effect on lipases production but the titers of more than 7 IU/ml were obtained in presence of peptone, glucose, olive oil, triton X and recycled oil. In a separate set of experiments, the utilization of recycled motor oil, wheat-bran and glycerol was studied. It was observed that the strain produced higher titers of lipases (>8 IU/ml) in presence of glycerol. Therefore, the strain TS16 may be used to produce lipases on inexpensive substrates.