Occurrence of 5′-deoxyadenosylcobalamin and its physiological function as the coenzyme of methylmalonyl-CoA mutase in a marine eukaryotic microorganism, Schizochytrium limacinum SR21

被引：5

作者：

Miyamoto, Emi ^{[1
]}

Tanfoka, Yuri ^{[2
]}

Yukino, Tsugiyo ^{[3
]}

Hayashi, Masahiro ^{[4
]}

Watanabe, Fumio ^{[5
]}

Nakano, Yoshihisa ^{[2
]}

机构：

[1] Nagasaki Int Univ, Dept Hlth & Nutr, Sasebo 8593298, Japan

[2] Osaka Prefecture Univ, Dept Appl Biol Chem, Sakai, Osaka 5995831, Japan

[3] Nayoro City Univ, Dept Nutr Sci, Nayoro 0968641, Japan

[4] Miyazaki Univ, Fac Agr, Dept Biol Product & Environm Sci, Miyazaki 8892192, Japan

[5] Tottori Univ, Fac Agr, Sch Agr Biol & Environm Sci, Tottori 6808553, Japan

来源：

JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY | 2007年 / 53卷 / 06期

关键词：

cobalamin; 5'-deoxyadenosylcobalamin; vitamin B-12; methylmalonyl-CoA mutase; Schizochytrium limacinum SR21;

D O I：

10.3177/jnsv.53.471

中图分类号：

R15 [营养卫生、食品卫生]; TS201 [基础科学];

学科分类号：

100403 ;

摘要：

A marine eukaryotic microorganism, Schizochytrium limacinum SR21, had the ability to absorb and accumulate exogenous cobalamin, which was converted to the cobalamin coenzymes 5'-deoxyadenosylcobalamin (20.10%) and methylcobalamin (29.6%). A considerably high activity (about 38 mU/mg protein) of 5'-deoxyadenosylcobalamin-dependent methylmalonyl-CoA mutase (EC 5.4.99.2) involved in amino acid and odd-chain fatty acid metabolism was found in the cell homogenate of S. Iimacinum SR21. The enzyme was purified to homogeneity and characterized.

引用

页码：471 / 475

页数：5