Reversible immobilization of lipase on phenylalanine containing hydrogel membranes

被引：38
作者：
Arica, MY [1 ]
Kaçar, Y
Ergene, A
Denizli, A
机构：
[1] Kirikkale Univ, Dept Biol, TR-71450 Yahsihan Krikkale, Turkey
[2] Hacettepe Univ, Dept Chem, TR-06532 Beytepe Ankara, Turkey
来源：
PROCESS BIOCHEMISTRY | 2001年 / 36卷 / 8-9期
关键词：
adsorption; immobilization; lipase; phenylalanine; poly(HEMA) membrane;
D O I：
10.1016/S0032-9592(00)00289-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Poly(2-hydroxyethylmethacrylate-co-methacrylamidophenlyalanine) poly(HEMA-MAPA) membranes were prepared by UV-initiated photopolymerization of HEMA and MAPA. Lipase immobilization onto these membranes from aqueous solutions containing different amounts of lipase at different pH was investigated in a batch system. The lipase adsorption capacity of the membranes was increased as the MAPA ratio increased in the membrane structure. The maximum lipase immobilization capacity of the poly(HEMA-MAPA-3) membrane was 135 mug cm(-2). The optimum temperature was 5 degreesC higher than that of the free enzyme and was significantly broader. The storage stability increased with immobilization. The enzyme could be repeatedly adsorbed and desorbed without any significant loss in adsorption capacity. (C) 2001 Elsevier Science Ltd. All rights reserved.
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页码：847 / 854
页数：8
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