Short Chain Fatty Acids (SCFA) Reprogram Gene Expression in Human Malignant Epithelial and Lymphoid Cells

被引：28

作者：

Astakhova, Lidiia ^{[1
,3
]}

Ngara, Mtakai ^{[2
]}

Babich, Olga ^{[1
]}

Prosekov, Aleksandr ^{[1
]}

Asyakina, Lyudmila ^{[1
]}

Dyshlyuk, Lyubov ^{[1
]}

Midtvedt, Tore ^{[3
]}

Zhou, Xiaoying ^{[3
]}

Ernberg, Ingemar ^{[3
]}

Matskova, Liudmila ^{[3
]}

机构：

[1] Inst Food Sci & Technol, Kemerovo, Russia

[2] Karolinska Inst, Ludwig Inst Canc Res, Dept Cell & Mol Biol CMB, Stockholm, Sweden

[3] Karolinska Inst, Dept Microbiol Tumor & Cell Biol MTC, Stockholm, Sweden

来源：

PLOS ONE | 2016年 / 11卷 / 07期

关键词：

EPSTEIN-BARR-VIRUS; HISTONE-DEACETYLASE ACTIVITY; SET ENRICHMENT ANALYSIS; IN-VITRO; INHIBITION; INDUCTION; ESTABLISHMENT; FERMENTATION; LYMPHOBLASTS; ACTIVATION;

D O I：

10.1371/journal.pone.0154102

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The effect of short chain fatty acids (SCFAs) on gene expression in human, malignant cell lines was investigated, with a focus on signaling pathways. The commensal microbial flora produce high levels of SCFAs with established physiologic effects in humans. The most abundant SCFA metabolite in the human microflora is n-butyric acid. It is well known to activate endogenous latent Epstein-Barr virus (EBV), that was used as a reference read out system and extended to EBV+ epithelial cancer cell lines. N-butyric acid and its salt induced inflammatory and apoptotic responses in tumor cells of epithelial and lymphoid origin. Epithelial cell migration was inhibited. The n-butyric gene activation was reduced by knockdown of the cell membrane transporters MCT-1 and -4 by siRNA. N-butyric acid show biologically significant effects on several important cellular functions, also with relevance for tumor cell phenotype.

引用

页数：18

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