Tuning of donor-acceptor linker in rhodamine-coumarin conjugates leads remarkable solvent dependent FRET efficiency for Al3+ imaging in HeLa cells

被引:21
作者
Adhikari, Susanta [1 ]
Mandal, Sandip [2 ]
Ghosh, Avijit [1 ]
Guria, Subhajit [1 ]
Das, Debasis [2 ]
机构
[1] Univ Calcutta, Dept Chem, 92 APC Rd, Kolkata 700009, W Bengal, India
[2] Univ Burdwan, Dept Chem, Burdwan 713104, W Bengal, India
关键词
Rhodamine-coumarin conjugates; Solvent dependent FRET; DFT studies; Cell imaging; RESONANCE ENERGY-TRANSFER; RATIOMETRIC FLUORESCENT-PROBE; OFF-ON; 2-STEP FRET; SENSOR; HG2+; CHEMOSENSOR; DESIGN; IONS; MOLECULE;
D O I
10.1016/j.snb.2016.04.135
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A series of rhodamine-coumarin conjugates with tunable linkers function as FRET based sensors for biological Al3+ imaging in living HeLa cells under fluorescence microscope. In methanol, all probes show chelation enhanced fluorescence (CHEF) assisted fluorescence resonance energy transfer (FRET) process. A remarkable change in FRET efficiency is observed with increasing water content of the media for a particular probe. DFT studies corroborate experimental observations. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码:222 / 230
页数:9
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