Directed evolution and synthetic biology applications to microbial systems

被引：50

作者：

Bassalo, Marcelo C. ^{[1
,2
]}

Liu, Rongming ^{[2
]}

Gill, Ryan T. ^{[2
]}

机构：

[1] Univ Colorado, Dept Mol Cellular & Dev Biol, Boulder, CO 80303 USA

[2] Univ Colorado, Dept Chem & Biol Engn, Boulder, CO 80303 USA

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2016年 / 39卷

关键词：

ESCHERICHIA-COLI; METABOLIC PATHWAYS; RNA DEVICES; CRISPR-CAS; E; COLI; GENOME; DESIGN; OPTIMIZATION; MUTAGENESIS; COMPLEX;

D O I：

10.1016/j.copbio.2016.03.016

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Biotechnology applications require engineering complex multi genic traits. The lack of knowledge on the genetic basis of complex phenotypes restricts our ability to rationally engineer them. However, complex phenotypes can be engineered at the systems level, utilizing directed evolution strategies that drive whole biological systems toward desired phenotypes without requiring prior knowledge of the genetic basis of the targeted trait. Recent developments in the synthetic biology field accelerates the directed evolution cycle, facilitating engineering of increasingly complex traits in biological systems. In this review, we summarize some of the most recent advances in directed evolution and synthetic biology that allows engineering of complex traits in microbial systems. Then, we discuss applications that can be achieved through engineering at the systems level.

引用

页码：126 / 133

页数：8

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