Aubergine is a component of a nanos mRNA localization complex

被引:31
作者
Becalska, Agata N. [1 ]
Kim, YoungJung R. [1 ]
Belletier, Nicolette G. [1 ]
Lerit, Dorothy A. [1 ]
Sinsimer, Kristina S. [1 ]
Gavis, Elizabeth R. [1 ]
机构
[1] Princeton Univ, Dept Mol Biol, Princeton, NJ 08544 USA
基金
加拿大自然科学与工程研究理事会;
关键词
Aubergine; nanos; Drosophila; mRNA localization; Rumplestiltskin; Segmentation; TRANSLATIONAL CONTROL ELEMENT; POLE CELL-FORMATION; DROSOPHILA-MELANOGASTER; OSKAR TRANSLATION; MATERNAL GENE; ACTIVATION; POSTERIOR; MUTATIONS; CHECKPOINT; OOGENESIS;
D O I
10.1016/j.ydbio.2010.10.002
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Localization of nanos (nos) mRNA to the posterior pole of the Drosophila oocyte is essential for abdominal segmentation and germline development during embryogenesis. Posterior localization is mediated by a complex cis-acting localization signal in the nos 3' untranslated region that comprises multiple partially redundant elements. Genetic analysis suggests that this signal is recognized by RNA-binding proteins and associated factors that package nos mRNA into a localization competent ribonucleoprotein complex. However, functional redundancy among localization elements has made the identification of individual localization factors difficult. Indeed, only a single direct-acting nos localization factor, Rumpelstiltskin (Rump), has been identified thus far. Through a sensitized genetic screen, we have now identified the Argonaute family member Aubergine (Aub) as a nos localization factor. Aub interacts with nos mRNA in vivo and co-purifies with Rump in an RNA-dependent manner. Our results support a role for Aub, independent of its function in RNA silencing, as a component of a nos mRNA localization complex. (C) 2010 Elsevier Inc. All rights reserved.
引用
收藏
页码:46 / 52
页数:7
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