Engineering molecular recognition in alkane oxidation catalysed by cytochrome P450cam

被引：20

作者：

Stevenson, JA ^{[1
]}

Bearpark, JK ^{[1
]}

Wong, LL ^{[1
]}

机构：

[1] Univ Oxford, Inorgan Chem Lab, Dept Chem, Oxford OX1 3QR, England

来源：

NEW JOURNAL OF CHEMISTRY | 1998年 / 22卷 / 06期

关键词：

D O I：

10.1039/a801637b

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The activity, selectivity and coupling efficiency of alkane oxidation by the monooxygenase enzyme cytochrome P450(cam) can be enhanced by engineering the active site topology to accommodate different alkanes: reducing the active site volume by the Y96F-V247L double mutations resulted in four-fold higher activity for the oxidation of hexane (1) than 3-methylpentane (2) while the larger active site of the Y96A-V247A double mutant gave rise to a two-fold preference for 2 over 1.

引用

页码：551 / 552

页数：2

共 12 条

[1] CALCULATED AND EXPERIMENTAL ABSOLUTE STEREOCHEMISTRY OF THE STYRENE AND BETA-METHYLSTYRENE EPOXIDES FORMED BY CYTOCHROME-P450(CAM) [J].

FRUETEL, JA ;

COLLINS, JR ;

CAMPER, DL ;

LOEW, GH ;

DEMONTELLANO, PRO .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1992, 114 (18) :6987-6993

[2] Engineering the selectivity of aliphatic C-H bond oxidation catalysed by cytochrome P450cam [J].

Jones, NE ;

England, PA ;

Rouch, DA ;

Wong, LL .

CHEMICAL COMMUNICATIONS, 1996, (21) :2413-2414

[3] RAPID AND EFFICIENT SITE-SPECIFIC MUTAGENESIS WITHOUT PHENOTYPIC SELECTION [J].

KUNKEL, TA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (02) :488-492

[4] MOLECULAR RECOGNITION IN CYTOCHROME-P-450 - MECHANISM FOR THE CONTROL OF UNCOUPLING REACTIONS [J].

LOIDA, PJ ;

SLIGAR, SG .

BIOCHEMISTRY, 1993, 32 (43) :11530-11538

[5] A CONSERVED RESIDUE OF CYTOCHROME-P-450 IS INVOLVED IN HEME-OXYGEN STABILITY AND ACTIVATION [J].

MARTINIS, SA ;

ATKINS, WM ;

STAYTON, PS ;

SLIGAR, SG .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1989, 111 (26) :9252-9253

[6]

MUELLER EJ, 1995, CYTOCHROME P 450 STR

[7] The catalytic activity of cytochrome P450(cam) towards styrene oxidation is increased by site-specific mutagenesis [J].

Nickerson, DP ;

HarfordCross, CF ;

Fulcher, SR ;

Wong, LL .

FEBS LETTERS, 1997, 405 (02) :153-156

[8]

PETERSON JA, 1990, J BIOL CHEM, V265, P6066

[9] The catalytic oxidation of linear and branched alkanes by cytochrome P450(cam) [J].

Stevenson, JA ;

Westlake, ACG ;

Whittock, C ;

Wong, LL .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1996, 118 (50) :12846-12847

[10]

UNGER BP, 1986, J BIOL CHEM, V261, P1158

← 1 2 →