Reversible and Highly Ordered Biointerfaces for Efficient Capture and Nondestructive Release of Circulating Tumor Cells

被引:14
|
作者
Wang, Siyi [1 ]
Cui, Jiasen [2 ]
Fan, Qian [1 ,3 ]
Gan, Jiaxing [2 ]
Liu, Chunran [2 ]
Wang, Yuanhe [5 ]
Yang, Ting [1 ]
Wang, Jianhua [1 ]
Yang, Chaoyong [3 ,4 ]
机构
[1] Northeastern Univ, Coll Sci, Res Ctr Analyt Sci, Dept Chem, Shenyang 110819, Peoples R China
[2] China Med Univ, Sch & Hosp Stomatol, Dept Oral Pathol, Liaoning Prov Key Lab Oral Dis, Shenyang 110001, Peoples R China
[3] Shanghai Jiao Tong Univ, Renji Hosp, Sch Med, Inst Mol Med, Shanghai 200127, Peoples R China
[4] Xiamen Univ, Coll Chem & Chem Engn, Dept Chem Biol, MOE Key Lab Spectrochem Anal & Instrumentat,State, Xiamen 361005, Peoples R China
[5] China Med Univ, Canc Hosp, Liaoning Canc Hosp & Inst, Dept Gastrointestinal Canc, Shenyang 110042, Peoples R China
关键词
HEPATOCELLULAR-CARCINOMA; LABEL-FREE; BORONATE;
D O I
10.1021/acs.analchem.2c01743
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The engineering strategy of artificial biointerfaces is vital for governing their performances in bioanalysis and diagnosis. Highly ordered arrangement of affinity ligands on the interface surface facilitates efficient interaction with target molecules, whereas biointerfaces aimed at drug delivery or rare cell isolation require sophisticated stimuliresponse mechanisms. However, it is still challenging to facilely fabricate biointerfaces possessing the two features. Herein, we endow a biointerface with both reversibility and capability to orderly assemble affinity ligands by introducing boronic acid moieties alone. By boronate conjugation via glycosylation sites, avidin was well arranged at the surface of boronic acid-decorated carbon nitride nanosheets for the assembly of biotinylated aptamers. The ordered orientation of aptamers largely relieved their inactivation caused by inter-strand entanglement, facilitating significant increase in cell affinity for the isolation of circulating tumor cells (CTCs). The reversible boronate conjugation also facilitated mild release of CTCs by acid fructose with high cell viability. This engineered interface was capable of isolating CTCs from the peripheral blood of tumor-bearing mice and cancer patients. The successful utilization of the isolated CTCs in the downstream drug susceptibility test and mutation analysis demonstrated the clinical potential of this biointerface for the early diagnosis of cancers and precision medicine.
引用
收藏
页码:9450 / 9458
页数:9
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