Effects of Pseudomonas aeruginosa on Microglial-Derived Extracellular Vesicle Biogenesis and Composition

被引：6
作者：
Jones, Leandra B. [1 ]
Kumar, Sanjay [2 ,3 ]
Bell, Courtnee' R. [1 ]
Peoples, Veolonda A. [1 ,4 ,5 ]
Crenshaw, Brennetta J. [1 ]
Coats, Mamie T. [1 ,4 ,5 ]
Scoffield, Jessica A. [6 ]
Rowe, Glenn C. [7 ]
Sims, Brian [2 ,3 ]
Matthews, Qiana L. [1 ,4 ]
机构：
[1] Alabama State Univ, Coll Sci Technol Engn & Math, Dept Biol Sci, Microbiol Program, Montgomery, AL 36104 USA
[2] Univ Alabama Birmingham, Div Neonatol, Dept Pediat, Birmingham, AL 35294 USA
[3] Univ Alabama Birmingham, Div Neonatol, Dept Cell Dev & Integrat Biol, Birmingham, AL 35294 USA
[4] Alabama State Univ, Coll Sci Technol Engn & Math, Dept Biol Sci, Montgomery, AL 36104 USA
[5] Alabama State Univ, Ctr NanoBiotechnol Res CNBR, Montgomery, AL 36104 USA
[6] Univ Alabama Birmingham, Sch Med, Dept Microbiol, Birmingham, AL 35294 USA
[7] Univ Alabama Birmingham, Dept Med, Div Cardiovasc Dis, Birmingham, AL 35294 USA
来源：
PATHOGENS | 2019年 / 8卷 / 04期
基金：
美国国家科学基金会; 美国国家卫生研究院;
关键词：
Extracellular vesicles; exosome; biogenesis; microglia; Pseudomonas aeruginosa; MOLECULAR CHAPERONES; TNF-ALPHA; EXOSOMES; INFECTIONS; MITOCHONDRIA; PATHOGENESIS; MECHANISMS; RESISTANCE; SECRETION; CYTOKINE;
D O I：
10.3390/pathogens8040297
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The packaging of molecular constituents inside extracellular vesicles (EVs) allows them to participate in intercellular communication and the transfer of biological molecules, however the role of EVs during bacterial infection is poorly understood. The goal of this study was to examine the effects of Pseudomonas aeruginosa (P. aeruginosa) infection on the biogenesis and composition of EVs derived from the mouse microglia cell line, BV-2. BV-2 cells were cultured in exosome-free media and infected with 0, 1.3 x 10(4), or 2.6 x 10(4) colony forming units per milliliter P. aeruginosa for 72 h. The results indicated that compared with the control group, BV-2 cell viability significantly decreased after P. aeruginosa infection and BV-2-derived EVs concentration decreased significantly in the P. aeruginosa-infected group. P. aeruginosa infection significantly decreased chemokine ligand 4 messenger RNA in BV-2-derived infected EVs, compared with the control group (p <= 0.05). This study also revealed that heat shock protein 70 (p <= 0.05) and heat shock protein 90 beta (p <= 0.001) levels of expression within EVs increased after P. aeruginosa infection. EV treatment with EVs derived from P. aeruginosa infection reduced cell viability of BV-2 cells. P. aeruginosa infection alters the expression of specific proteins and mRNA in EVs. Our study suggests that P. aeruginosa infection modulates EV biogenesis and composition, which may influence bacterial pathogenesis and infection.
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页数：15
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