Occurrence and Genetic Characteristics of Cryptosporidium hominis and Cryptosporidium andersoni in Horses from Southwestern China

被引：22

作者：

Deng, Lei ^{[1
]}

Li, Wei ^{[1
]}

Zhong, Zhijun ^{[1
]}

Gong, Chao ^{[1
]}

Cao, Xuefeng ^{[1
]}

Song, Yuan ^{[1
]}

Wang, Wuyou ^{[1
]}

Huang, Xiangming ^{[2
]}

Liu, Xuehan ^{[1
]}

Hu, Yanchun ^{[1
]}

Fu, Hualin ^{[1
]}

He, Min ^{[1
]}

Wang, Ya ^{[1
]}

Zhang, Yue ^{[2
]}

Wu, Kongju ^{[2
]}

Peng, Guangneng ^{[1
]}

机构：

[1] Sichuan Agr Univ, Coll Vet Med, Key Lab Anim Dis & Human Hlth Sichuan Prov, Chengdu 611130, Sichuan, Peoples R China

[2] Chengdu Giant Panda Breeding Res Base, Chengdu 625001, Sichuan, Peoples R China

来源：

JOURNAL OF EUKARYOTIC MICROBIOLOGY | 2017年 / 64卷 / 05期

关键词：

GP60; multilocus sequence typing; transmission; ENTEROCYTOZOON-BIENEUSI; DAIRY CALVES; IDENTIFICATION; PARASITES; GENOTYPE; SPP; FOALS; TRANSMISSION; CHILDREN; DONKEYS;

D O I：

10.1111/jeu.12399

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A total of 333 fecal specimens from horses in southwestern China were genotyped based on analysis of the small subunit rRNA (SSU rRNA) gene. Cryptospoodium hominis and Cryptosporidium andersoni were identified in 2 and 4 stool specimens, respectively. The identification of C. hominis was confirmed by sequence analysis of the 70-kDa heat shock protein (HSP70) and oocyst wall protein (COWP) genes. Subtyping analysis of the 60-kDa glycoprotein (GP60) gene sequence of C. hominis revealed a new rare subtype Id, named IdA15; only three Id isolates have been reported in humans to date. Multilocus sequence typing (MLST) analysis indicated that the C. andersoni subtype was A6, A5, A2, and A1 at the four minisatellite loci (MS1, MS2, MS3, and MS16, respectively). This is the first report to identify the presence of C. andersoni and C. hominis in horses in southwestern China and the first to identify a rare zoonotic subtype Id of C. hominis in horses. These findings suggest that infected horses may act as potential reservoirs of Cryptosporidium to transmit infections to humans.

引用

页码：716 / 720

页数：5

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