High-Resolution AFM Imaging of Prestin Purified and Reconstituted into an Artificial Lipid Bilayer

被引：1

作者：

Wada, Hiroshi ^{[1
]}

Kumano, Shun ^{[1
]}

Murakoshi, Michio ^{[1
]}

Iida, Koji ^{[1
]}

Hamana, Hiroshi ^{[1
]}

机构：

[1] Tohoku Univ, Dept Bioengn & Robot, Sendai, Miyagi 980, Japan

来源：

WHAT FIRE IS IN MINE EARS: PROGRESS IN AUDITORY BIOMECHANICS: PROCEEDINGS OF THE 11TH INTERNATIONAL MECHANICS OF HEARING WORKSHOP | 2011年 / 1403卷

关键词：

prestin; reconstitution; atomic force microscopy; OUTER HAIR-CELLS; ATOMIC-FORCE MICROSCOPY; MOTOR PROTEIN PRESTIN; MEMBRANE-PROTEINS; PLASMA-MEMBRANE; DENSITY;

D O I：

10.1063/1.3658084

中图分类号：

R36 [病理学]; R76 [耳鼻咽喉科学];

学科分类号：

100104 ; 100213 ;

摘要：

In the present study, purified prestin was observed by atomic force microscopy (AFM). First, the lipid bilayer was formed on a freshly cleaved mica disk by the deposition of small unilamellar lipid vesicles. Such bilayer was destabilized by incubation with a detergent. Afterward, purified prestin was added to the destabilized lipid bilayer. Finally, a high resolution image of the prestin-reconstituted lipid bilayer was acquired by AFM. As a result, densely embedded prestin with a diameter of 11.0 +/- 1.3 nm was visualized. From the obtained image, cytoplasmic side of prestin was found to form a ring-like structure with four peaks and one valley at its center.

引用

页数：6

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