Multidrug-Resistant Methicillin-Resistant Coagulase-Negative Staphylococci in Healthy Poultry Slaughtered for Human Consumption

被引：28

作者：

Silva, Vanessa ^{[1
,2
,3
,4
]}

Canica, Manuela ^{[5
,6
]}

Ferreira, Eugenia ^{[5
,6
]}

Vieira-Pinto, Madalena ^{[7
]}

Saraiva, Candido ^{[7
]}

Pereira, Jose Eduardo ^{[1
,7
,8
]}

Capelo, Jose Luis ^{[9
,10
]}

Igrejas, Gilberto ^{[2
,3
,4
]}

Poeta, Patricia ^{[1
,4
,7
,8
]}

机构：

[1] Univ Tras Os Montes & Alto Douro UTAD, Dept Vet Sci, Microbiol & Antibiot Resistance Team MicroART, P-5000801 Vila Real, Portugal

[2] Univ Tras Os Montes & Alto Douro, Dept Genet & Biotechnol, P-5000801 Vila Real, Portugal

[3] Univ Tras Os Montes & Alto Douro UTAD, Funct Genom & Prote Unit, P-5000801 Vila Real, Portugal

[4] Univ Nova Lisboa, NOVA Sch Sci & Technol, Dept Chem, LAQV REQUIMTE, P-2829516 Caparica, Portugal

[5] Natl Inst Hlth Dr Ricardo Jorge, Dept Infect Dis, Natl Reference Lab Antibiot Resistances & Healthc, Av Padre Cruz, P-1649016 Lisbon, Portugal

[6] Univ Porto, Ctr Studies Anim Sci, Inst Agr & Agrifood Sci & Technol, P-4051401 Oporto, Portugal

[7] Univ Tras Os Montes & Alto Douro UTAD, CECAV Vet & Anim Res Ctr, P-5000801 Vila Real, Portugal

[8] Associate Lab Anim & Vet Sci AL4AnimalS, P-5000801 Vila Real, Portugal

[9] NOVA Univ Lisbon, Fac Sci & Technol, Chem Dept, LAQV REQUIMTE,BIOSCOPE Grp, P-2825466 Almada, Portugal

[10] Proteomass Sci Soc, P-2825466 Costa De Caparica, Portugal

来源：

ANTIBIOTICS-BASEL | 2022年 / 11卷 / 03期

关键词：

coagulase-negative Staphylococcus; CoNS; antimicrobial resistance; poultry; quails; broilers; ANTIBIOTIC-RESISTANCE; FUSIDIC ACID; GENE CFR; AUREUS; IDENTIFICATION; PREVALENCE; PIGS; LINCOSAMIDES; DETERMINANTS; ENTEROCOCCI;

D O I：

10.3390/antibiotics11030365

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Coagulase-negative staphylococci are commensals that are known to be prevalent in most environments, and they are also an important reservoir of antimicrobial-resistant genes. Staphylococcal infections in animal husbandry are a high economic burden. Thus, we aimed to determine the prevalence and species diversity of methicillin-resistant coagulase-negative staphylococci (MRCoNS) in poultry slaughtered for human consumption and to study the antimicrobial resistance of the isolates. Swab samples were recovered from 220 commercial chickens, homebred chickens and quails. Species identification was performed using MALDI-TOF. Antimicrobial susceptibility testing was performed by the disc diffusion method against 14 antimicrobials. The presence of antimicrobial-resistant genes was investigated by polymerase chain reaction. Totals of 11 (19.6%), 13 (20.3%), and 51 (51%) MRCoNS were isolated from commercial chickens, homebred chickens and quails, respectively. S. lentus was isolated from all homebred chickens, whereas 11 S. lentus and 2 S. urealyticus were isolated from commercial chickens. As for quails, the most prevalent MRCoNS were S. urealyticus. Almost all isolates had a multidrug-resistant profile and carried the mecA gene. Most isolates showed resistance to erythromycin, clindamycin, penicillin, tetracycline, ciprofloxacin and fusidic acid and harbored the ermA, ermB, ermC, mphC tetK, tetL, tetM and tetO genes. This study showed a frequent occurrence of multidrug resistance in MRCoNS isolated from healthy poultry in Portugal.

引用

页数：11

共 71 条

[1] Antimicrobial susceptibility and presence of resistance genes in staphylococci from poultry [J].