3D inverted colloidal crystals in realistic cell migration assays for drug screening applications

被引：10

作者：

da Silva, Joakim ^{[1
]}

Lautenschlaeger, Franziska ^{[1
]}

Kuo, Cheng-Hwa R. ^{[1
]}

Guck, Jochen ^{[1
]}

Sivaniah, Easan ^{[1
]}

机构：

[1] Univ Cambridge, Cavendish Lab, Biol & Soft Sci Sect, Cambridge CB3 0HE, England

来源：

INTEGRATIVE BIOLOGY | 2011年 / 3卷 / 12期

基金：

英国工程与自然科学研究理事会;

关键词：

ACUTE PROMYELOCYTIC LEUKEMIA; SCAFFOLDS; HYDROGELS; MATRICES; ELASTICITY;

D O I：

10.1039/c1ib00065a

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Screening drugs for their specific impact on cell mechanics, in addition to targeting adhesion and proteolysis, will be important for successfully moderating migration in infiltrative disorders including cancer metastasis. We present 3D inverted colloidal crystals made of hydrogel as a realistic cell migration assay, where the geometry and stiffness can be set independently to mimic the tissue requirements in question. We show the utility of this 3D assay for drug screening purposes, specifically in contrast to conventional 2D migration studies, by surveying the effects of commonly used cytoskeletal toxins that impact cell mechanics. This assay allows studying large cell numbers for good statistics but at single-cell resolution.

引用

页码：1202 / 1206

页数：5

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