Validation of a polymerase chain reaction-oligochromatovaphy test for detection of influenza A (H1N1) 2009 virus

被引：2

作者：

Fuenzalida, Loreto ^{[1
]}

Prat, Cristina ^{[1
,2
]}

Blanco, Silvia ^{[1
,2
]}

Bas, Albert ^{[2
]}

Dominguez, M. J. ^{[3
]}

Hernandez, M. Angeles ^{[1
]}

Rodrigo, Carlos ^{[4
]}

Ausina, Vicente ^{[1
,2
]}

机构：

[1] Univ Autonoma Barcelona, Dept Genet & Microbiol, Inst Invest Ciences Salut Germans Trias & Pujol, Serv Microbiol,Hosp Univ Germans Trias & Pujol, Badalona 08916, Spain

[2] Inst Salud Carlos III, CIBER Enfermedades Respiratorias, Madrid 28220, Spain

[3] Hosp Badalona Germans Trias & Pujol, Inst Invest Ciences Salut Germans Trias & Pujol, Servei Urgencies, Badalona 08216, Spain

[4] Hosp Badalona Germans Trias & Pujol, Inst Invest Ciences Salut Germans Trias & Pujol, Servei Pediat, Badalona 08216, Spain

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2012年 / 72卷 / 02期

关键词：

Influenza A (H1N1) 2009; Pandemic; Oligochromatography; Real-time RT-PCR; Rapid detection; RESPIRATORY VIRUSES; AMPLIFICATION; DIAGNOSIS; ASSAYS;

D O I：

10.1016/j.diagmicrobio.2011.10.007

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The outbreak of pandemic influenza A (H1N1) 2009 virus caused the first influenza pandemic disease of the 21st century. In August 2010, the pandemic moved into the post-pandemic period. However, localized outbreaks of various magnitudes continued with a higher rate of disease severity. The aim of this study was to assess a new polymerase chain reaction (PCR)-oligochromatographic assay (Speed-Oligo) in the diagnosis of novel influenza A (H1N1) 2009. A total of 405 nasopharyngeal aspirate specimens from 400 pediatric and adults patients with suspected infection of pandemic influenza A (H1N1) 2009 were analyzed. The sensitivity and specificity values of the Speed-Oligo assay in comparison to reverse transcriptase PCR assay developed by the Centers for Disease Control and Prevention were 86.5% and 92.2%, respectively. The new assay is simple, rapid, and provides a good sensitivity for detection of influenza A (H1N1) 2009. This assay might be a good alternative to real-time PCR assays for laboratories not equipped with real-time PCR instruments. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：144 / 149

页数：6

共 20 条

[1]

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[2]

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[3]

Centers for Disease Control and Prevention (CDC), 2009, MMWR Morb Mortal Wkly Rep, V58, P1029

[4] Evaluation of New Rapid Antigen Test for Detection of Pandemic Influenza A/H1N1 2009 Virus [J].