Apoptosis-Mediated Inhibition of Human Breast Cancer Cell Proliferation by Lemon Citrus Extract

被引：0

作者：

Alshatwi, Ali A. ^{[1
]}

Shafi, Gowhar ^{[1
]}

Hasan, Tarique N. ^{[1
]}

Al-Hazzani, Amal A. ^{[2
]}

Alsaif, Mohammed A. ^{[3
]}

Alfawaz, Mohammed A. ^{[1
]}

Lei, K. Y. ^{[4
]}

Munshi, Anjana ^{[5
]}

机构：

[1] King Saud Univ, Coll Food & Agr Sci, Dept Food Sci & Nutr, Mol Canc Biol Res Lab, Riyadh, Saudi Arabia

[2] King Saud Univ, Coll Sci, Dept Bot & Microbiol, Riyadh 11451, Saudi Arabia

[3] King Saud Univ, Coll Appl Med Sci, Riyadh, Saudi Arabia

[4] Univ Maryland, College Pk, MD 20742 USA

[5] Inst Genet & Hosp Genet Dis, Hyderabad, Andhra Pradesh, India

来源：

ASIAN PACIFIC JOURNAL OF CANCER PREVENTION | 2011年 / 12卷 / 06期

关键词：

Lemon citrus; apoptosis; MCF-7; cells; gene expression; mitochondrial membrane potential; BAX; P53; DEATH; BCL-2; PROTEINS;

D O I：

暂无

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Dietary phytochemicals have a variety of antitumor properties. In the present study, the antitumor activity of methanolic extract of lemon fruit (lemon extract; LE) (LE) on the MCF-7 breast cancer cell line was investigated in vitro. Apoptotic cell death was analyzed using the TUNEL assay. In addition, the apoptosis mediated by LE extract in the MCF-7 cells was associated with the increased expression of the tumor suppressor p53 and caspase-3. Additionally, the expression of a pro-apoptotic gene, bax, was increased, and the expression of an anti-apoptotic gene, bcl-2, was decreased by LE extract treatment, resulting in a shift in the Bax: Bcl-2 ratio to one that favored apoptosis. The expression of a major apoptotic gene, caspase-3, was increased by LE extract treatment. In light of the above results, we concluded that LE extract can induce the apoptosis of MCF-7 breast cancer cells via Bax-related caspase-3 activation. This study provides experimental data that are relevant to the possible future clinical use of LE to treat breast cancer.

引用

页码：1555 / 1559

页数：5

共 24 条

[1] Insulin-like growth factor-binding protein-3 modulates expression of Bax and Bcl-2 and potentiates p53-independent radiation-induced apoptosis in human breast cancer cells [J].