The role of the proline-rich region in A1-type myosin essential light chains: Implications for information transmission in the actomyosin complex

被引：22

作者：

Timson, DJ ^{[1
]}

Trayer, IP ^{[1
]}

机构：

[1] UNIV BIRMINGHAM,SCH BIOCHEM,BIRMINGHAM B15 2TT,W MIDLANDS,ENGLAND

来源：

FEBS LETTERS | 1997年 / 400卷 / 01期

基金：

英国惠康基金;

关键词：

muscle contraction; actin binding; proline; protein engineering; ATPase; protein-protein interaction;

D O I：

10.1016/S0014-5793(96)01314-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The proline-rich region of A1-type myosin essential light chains functions as a spacer arm separating an actin binding site at the extreme N-terminus from the remainder of the protein, Alteration of the length of this region leaving the actin binding site intact results in altered actin-activated MgATPase kinetics when these light chains are hybridised into myosin subfragment-1. In the case of a mutant in which the length of the proline-rich region was doubled, actin binding by the light chain was uncoupled from kinetic modulation. The implications of this result for information transmission in the actomyosin complex are discussed.

引用

页码：31 / 36

页数：6

共 35 条

[1] CONFORMATIONAL CALCULATIONS ON THE ALA14-PRO27 LC1 SEGMENT OF RABBIT SKELETAL MYOSIN [J].

ABILLON, E ;

BREMIER, L ;

CARDINAUD, R .

BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1037 (03) :394-400

[2] THE BIOCHEMICAL BASIS OF THE REGULATION OF SMOOTH-MUSCLE CONTRACTION [J].

ALLEN, BG ;

WALSH, MP .

TRENDS IN BIOCHEMICAL SCIENCES, 1994, 19 (09) :362-368

[3] BINDING OF HEAVY-CHAIN AND ESSENTIAL LIGHT-CHAIN-1 OF S1 TO ACTIN DEPENDS ON THE DEGREE OF SATURATION OF F-ACTIN FILAMENTS WITH S1 [J].

ANDREEV, OA ;

BOREJDO, J .

BIOCHEMISTRY, 1995, 34 (45) :14829-14833

[4] A NOVEL HUMAN MYOSIN ALKALI LIGHT CHAIN IS DEVELOPMENTALLY REGULATED - EXPRESSION IN FETAL CARDIAC AND SKELETAL-MUSCLE AND IN ADULT ATRIA [J].

ARNOLD, HH ;

LOHSE, P ;

SEIDEL, U ;

BOBER, E .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1988, 178 (01) :53-60

[5] H-1-NMR STUDY OF MOBILITY AND CONFORMATIONAL CONSTRAINTS WITHIN THE PROLINE-RICH N-TERMINAL OF THE LC1 ALKALI LIGHT CHAIN OF SKELETAL MYOSIN - CORRELATION WITH SIMILAR SEGMENTS IN OTHER PROTEIN SYSTEMS [J].

BHANDARI, DG ;

LEVINE, BA ;

TRAYER, IP ;

YEADON, ME .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1986, 160 (02) :349-356

[6] STRUCTURE AND FUNCTION OF X-PRO DIPEPTIDE REPEATS IN THE TONB PROTEINS OF SALMONELLA-TYPHIMURIUM AND ESCHERICHIA-COLI [J].

BREWER, S ;

TOLLEY, M ;

TRAYER, IP ;

BARR, GC ;

DORMAN, CJ ;

HANNAVY, K ;

HIGGINS, CF ;

EVANS, JS ;

LEVINE, BA ;

WORMALD, MR .

JOURNAL OF MOLECULAR BIOLOGY, 1990, 216 (04) :883-895

[7] STRUCTURAL CHARACTERIZATION OF THE BETA-BEND RIBBON SPIRAL - CRYSTALLOGRAPHIC ANALYSIS OF 2 LONG (L-PRO-AIB)N SEQUENTIAL PEPTIDES [J].

DIBLASIO, B ;

PAVONE, V ;

SAVIANO, M ;

LOMBARDI, A ;

NASTRI, F ;

PEDONE, C ;

BENEDETTI, E ;

CRISMA, M ;

ANZOLIN, M ;

TONIOLO, C .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1992, 114 (16) :6273-6278

[8]

Fiske CH, 1925, J BIOL CHEM, V66, P375

[9]

FRANK G, 1974, EUR J BIOCHEM, V44, P317

[10] MASS-SPECTROMETRY OF PEPTIDES AND PROTEINS BY MATRIX-ASSISTED ULTRAVIOLET-LASER DESORPTION IONIZATION [J].

HILLENKAMP, F ;

KARAS, M .

METHODS IN ENZYMOLOGY, 1990, 193 :280-295

← 1 2 3 4 →