Structural biology of the Gram-negative bacterial conjugation systems

被引：92

作者：

Ilangovan, Aravindan ^{[1
]}

Connery, Sarah ^{[1
]}

Waksman, Gabriel ^{[1
]}

机构：

[1] UCL & Birkbeck, Inst Struct & Mol Biol, London WC1E 7HX, England

来源：

TRENDS IN MICROBIOLOGY | 2015年 / 23卷 / 05期

基金：

英国惠康基金;

关键词：

bacterial conjugation; DNA transport; substrate secretion; Type IV secretion system; T4SS; structural biology; IV SECRETION SYSTEM; CAG PATHOGENICITY ISLAND; T-PILUS BIOGENESIS; VIRB4; C-TERMINUS; AGROBACTERIUM-TUMEFACIENS; PLASMID R388; HELICOBACTER-PYLORI; CORE COMPLEX; DNA TRANSFER; PERIPLASMIC SURFACE;

D O I：

10.1016/j.tim.2015.02.012

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Conjugation, the process by which plasmid DNA is transferred from one bacterium to another, is mediated by type IV secretion systems (T4SSs). T4SSs are versatile systems that can transport not only DNA, but also toxins and effector proteins. Conjugative T4SSs comprise 12 proteins named VirB1-11 and VirD4 that assemble into a large membrane-spanning exporting machine. Before being transported, the DNA substrate is first processed on the cytoplasmic side by a complex called the relaxosome. The substrate is then targeted to the T4SS for export into a recipient cell. In this review, we describe the recent progress made in the structural biology of both the relaxosome and the T4SS.

引用

页码：301 / 310

页数：10

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