Fatty Acid Composition, Functional Group Analysis and Antioxidant Activity of Nymphia alba and Lupinus polyphyllus Seed Extracts

Seed extracts of Nymphia alba Linn. and Lupinus polyphyllus Lindl. were analyzed for fatty acid composition, functional group analysis and antioxidant activity. The petroleum ether extract of seeds were found dominant in unsaturated fatty acids with oleic acid (39.9%) and linoleic acid (29.6%) in L. polyphyllus and linoleic (37.5%) and oleic acid (10.9%) in N. alba. All the defatted seed extracts of N. alba and L. polyphyllus found to have powerful DPPH, ABTS, H2O2 and NBT antioxidant radical scavenging activity with reference to butylated hydroxy toluene (BHT). The defatted seed extracts were further analyzed with functional group analysis through FTIR found to contain numerous functional groups which may be responsible for their antioxidant activity.