Flocculation Properties of Poly(γ-Glutamic Acid) Produced from Bacillus subtilis Isolate

被引：46

作者：

Bajaj, Ishwar B. ^{[1
]}

Singhal, Rekha S. ^{[1
]}

机构：

[1] Univ Bombay, Food Engn & Technol Dept, Inst Chem Technol, Bombay 400019, Maharashtra, India

来源：

FOOD AND BIOPROCESS TECHNOLOGY | 2011年 / 4卷 / 05期

关键词：

Poly(gamma-glutamic acid); Bacillus subtilis; Flocculating activity; Bioflocculant; Marine bacteria; WASTE-WATER TREATMENT; BIOPOLYMER FLOCCULANT; MICROBIAL FLOCCULANT; MEDIUM OPTIMIZATION; GLUTAMIC ACID; LICHENIFORMIS; MUCILAGINOSUS; BIOSYNTHESIS; CULTURE;

D O I：

10.1007/s11947-009-0186-y

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Bacillus subtilis R 23 produced extracellular biopolymer showing excellent flocculation activity. The biopolymer was confirmed as poly(gamma-glutamic acid) (PGA) using high-performance liquid chromatography profile and product characterization. The production, characteristics, and flocculation properties of PGA were studied. PGA produced by B. subtilis R 23 was devoid of any polysaccharides and had a molecular weight of 6.2 x 10(6) Da. The flocculating activity of PGA in the kaolin suspension was markedly stimulated by the addition of bivalent and trivalent cations in optimum concentration. The pH of reaction mixture also influenced the flocculating activity. Response surface methodology was used to establish the optimum parameters for maximum flocculating activity and to study their interactions. A maximum flocculating activity of 30.32 +/- 1.4 1/optical density was obtained with 7.5 mg/L of PGA in combination with 8.0 mM of Ca(+2) at pH 7.5.

引用

页码：745 / 752

页数：8

共 28 条

[1] Isolation of Bacillus subtilis (chungkookjang), a poly-γ-glutamate producer with high genetic competence [J].

Ashiuchi, M ;

Kamei, T ;

Baek, DH ;

Shin, SY ;

Sung, MH ;

Soda, K ;

Yagi, T ;

Misono, H .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2001, 57 (5-6) :764-769

[2] Optimization of Aspergillus niger Fermentation for the Production of Glucose Oxidase [J].

Bankar, Sandip B. ;

Bule, Mahesh V. ;

Singhal, Rekha S. ;

Ananthanarayan, Laxmi .

FOOD AND BIOPROCESS TECHNOLOGY, 2009, 2 (04) :344-352

[3] Medium optimization by response surface methodology for poly-γ-glutamic acid production using dairy manure as the basis of a solid substrate [J].

Chen, X ;

Chen, SW ;

Sun, M ;

Yu, ZN .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2005, 69 (04) :390-396

[4]

Cromwick AM, 1996, BIOTECHNOL BIOENG, V50, P222, DOI 10.1002/(SICI)1097-0290(19960420)50:2<222::AID-BIT10>3.0.CO

[5]

2-P

[6] Characteristics of a bioflocculant produced by Bacillus mucilaginosus and its use in starch wastewater treatment [J].

Deng, SB ;

Bai, RB ;

Hu, XM ;

Luo, Q .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2003, 60 (05) :588-593

[7] A COLORIMETRIC METHOD FOR THE DETERMINATION OF SUGARS [J].

DUBOIS, M ;

GILLES, K ;

HAMILTON, JK ;

REBERS, PA ;

SMITH, F .

NATURE, 1951, 168 (4265) :167-167

[8] Bioflocculant production by culture of Serratia ficaria and its application in wastewater treatment [J].

Gong, Wen-Xin ;

Wang, Shu-Guang ;

Sun, Xue-Fei ;

Liu, Xian-Wei ;

Yue, Qin-Yan ;

Gao, Bao-Yu .

BIORESOURCE TECHNOLOGY, 2008, 99 (11) :4668-4674

[9] BIOSYNTHESIS AND HYDROLYSIS OF POLY(GAMMA-GLUTAMIC ACID) FROM BACILLUS-SUBTILIS IFO3335 [J].

GOTO, A ;

KUNIOKA, M .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1992, 56 (07) :1031-1035

[10] Transfer of Natrialba asiatica B1T to Natrialba taiwanensis sp nov and description of Natrialba aegyptiaca sp nov., a novel extremely halophilic, aerobic, non-pigmented member of the Archaea from Egypt that produces extracellular poly(glutamic acid) [J].

Hezayen, FF ;

Rehm, BHA ;

Tindall, BJ ;

Steinbüchel, A .

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2001, 51 :1133-1142

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