A reversed-phase high-performance liquid chromatographic method for the determination of aloesin, aloeresin a and anthraquinone in Aloe ferox

被引：19

作者：

Zahn, Michael ^{[1
]}

Trinh, Thao ^{[1
]}

Jeong, Mijeong Lee ^{[1
]}

Wang, Desheng ^{[1
]}

Abeysinghe, Padmapriya ^{[1
]}

Jia, Qi ^{[1
]}

Ma, Wenwen ^{[1
]}

机构：

[1] Unigen, Lacey, WA 98516 USA

来源：

PHYTOCHEMICAL ANALYSIS | 2008年 / 19卷 / 02期

关键词：

HPLC; method validation; Aloe ferox; aloesin; aloeresin a; barbaloin;

D O I：

10.1002/pca.1024

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A reversed-phase HPLC method for the quantification of aloesin, aloeresin a and anthraquinone (as barbaloin) in Aloe ferox Miller and aloe-related products has been developed and validated. The method utilized a C-18 column. with a water-methanol gradient and UV detection at 297 nm. The method validation included linearity, accuracy, precision, limit of detection, limit of quantitation, specificity and standard solution stability. The method showed good linearity (r > 0.99 for all components) and recovery (>85% for all components). The detection and quantitation limits for barbaloin were determined to be 0.02 and 0.1 ppm at signal-to-noise ratios of approximately 3: 1 and 10: 1, respectively. Copyright (C) 2007 John Wiley & Sons, Ltd.

引用

页码：122 / 126

页数：5

共 13 条

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