Impedance-Based Single-Cell Pipetting

被引：3

作者：

Bonzon, David ^{[1
,2
,3
]}

Muller, Georges ^{[2
,3
,4
]}

Bureau, Jean-Baptiste ^{[4
]}

Uffer, Nicolas ^{[2
,4
]}

Beuchat, Nicolas ^{[1
]}

Barrandon, Yann ^{[4
,5
,6
]}

Renaud, Philippe ^{[1
]}

机构：

[1] Ecole Polytech Fed Lausanne, Lab Microsyst 4, IMT, STI, Lausanne, VD, Switzerland

[2] SEED Biosci SA, Epalinges, Switzerland

[3] SEED Biosci SA, Renens, Switzerland

[4] Ecole Polytech Fed Lausanne, Lab Stem Cell Dynam, IBI, Lausanne, VD, Switzerland

[5] ASTAR, Inst Med Biol, Duke NUS Grad Med Sch, Singapore, Singapore

[6] Singapore Gen Hosp, Dept Plast Reconstruct & Aesthet Surg, Singapore, Singapore

来源：

SLAS TECHNOLOGY | 2020年 / 25卷 / 03期

基金：

瑞士国家科学基金会;

关键词：

single cell; dispensing; disposable pipet tip; impedance spectroscopy; EXPRESSION; STRATEGY;

D O I：

10.1177/2472630320911636

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Many biological methods are based on single-cell isolation. In single-cell line development, the gold standard involves the dilution of cells by means of a pipet. This process is time-consuming as it is repeated over several weeks to ensure clonality. Here, we report the modeling, designing, and testing of a disposable pipet tip integrating a cell sensor based on the Coulter principle. We investigate, test, and discuss the effects of design parameters on the sensor performances with an analytical model. We also describe a system that enables the dispensing of single cells using an instrumented pipet coupled with the sensing tip. Most importantly, this system allows the recording of an impedance trace to be used as proof of single-cell isolation. We assess the performances of the system with beads and cells. Finally, we show that the electrical detection has no effect on cell viability.

引用

页码：222 / 233

页数：12