Water-soluble polysaccharide from Erythronium sibiricum bulb: Structural characterisation and immunomodulating activity

被引：23
作者：
Kasimu, Rena [1 ]
Chen, Chunli [1 ]
Xie, Xiangyun [1 ]
Li, Xue [2 ]
机构：
[1] Xinjiang Med Univ, Pharm Coll, Urumqi 830011, Peoples R China
[2] Supervis & Testing Ctr Qual & Safety Agriprod Xin, Urumqi 830049, Peoples R China
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2017年 / 105卷
关键词：
Erythronium sibiricum; Polysaccharide structure; Immunomodulating activity; CHRYSANTHEMUM-MORIFOLIUM FLOWERS; ANTIOXIDANT ACTIVITY; ANTICOAGULANT ACTIVITY; PURIFICATION; MACROPHAGES; EXTRACTION; ANTITUMOR; CELLS;
D O I：
10.1016/j.ijbiomac.2017.07.060
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A water-soluble polysaccharide named ESBP2-1 was isolated from Erythronium sibiricum bulb through anion-exchange and size-exclusion chromatography. ESBP2-1 is a neutral polysaccharide fraction with an average molecular weight of 9.4 x 105 Da and is composed of glucose, galactose and arabinose in a ratio of 24.3:1.1:1. Methylation analysis, partial hydrolysis and NMR studies revealed that the backbone of ESBP2-1 primarily consists of repeating) -> 1)-alpha-D-Glcp-(4 -> units where the disaccharide side chains of Arap-(1 -> 6)-alpha-D-Galp-(1 -> residue are attached to the O-6 position of Glc. An in vitro assay showed that ESBP2-1 significantly promoted the proliferation and neutral red phagocytosis of RAW 264.7 macrophage cells. In addition, ESBP2-1 stimulated the production of secretory molecules (nitric oxide, TNF-alpha and IL-1 beta) of RAW264.7 cells in a dose-dependent manner. These results suggest that ESBP2-1 is a potential immunostimulator. (C) 2017 Published by Elsevier B.V.
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收藏
页码：452 / 462
页数：11
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