Long-term stability and protection efficacy of the RBD-targeting COVID-19 mRNA vaccine in nonhuman primates

被引：35

作者：

Zhao, Hui ^{[1
]}

Wang, Tie-Cheng ^{[2
]}

Li, Xiao-Feng ^{[1
]}

Zhang, Na-Na ^{[1
,3
]}

Li, Liang ^{[2
]}

Zhou, Chao ^{[1
]}

Deng, Yong-Qiang ^{[1
]}

Cao, Tian-Shu ^{[1
]}

Yang, Guan ^{[4
]}

Li, Rui-Ting ^{[1
]}

Huang, Yi-Jiao ^{[1
]}

Li, Yuan-Guo ^{[2
]}

Zhang, Yi-Ming ^{[5
]}

Li, Fang-Xu ^{[5
]}

Zhou, Yu-Ren ^{[6
]}

Jiang, Yu-Hang ^{[6
]}

Lu, Xi-Shan ^{[6
]}

Sun, Shi-Hui ^{[1
]}

Cheng, Meng-Li ^{[1
]}

Gu, Kai-Ping ^{[6
]}

Zhang, Mei ^{[6
]}

Ma, Qing-Qing ^{[1
]}

Yang, Xiao ^{[4
]}

Ying, Bo ^{[6
]}

Gao, Yu-Wei ^{[2
]}

Qin, Cheng-Feng ^{[1
,3
,7
]}

机构：

[1] Acad Mil Med Sci, Beijing Inst Microbiol & Epidemiol, State Key Lab Pathogen & Biosecur, Beijing 100071, Peoples R China

[2] Key Lab Jilin Prov Zoonosis Prevent & Control, Changchun 130122, Peoples R China

[3] Tsinghua Univ, Sch Med, Beijing 100084, Peoples R China

[4] Beijing Inst Life, Natl Ctr Prot Sci Beijing, Beijing Proteome Res Ctr, State Key Lab Prote, Beijing 102206, Peoples R China

[5] Shandong Normal Univ, Jinan 250014, Peoples R China

[6] Suzhou Abogen Biosci, Suzhou 215123, Peoples R China

[7] Chinese Acad Med Sci, Res Unit Discovery & Tracing Nat Focus Dis, Beijing 100071, Peoples R China

来源：

SIGNAL TRANSDUCTION AND TARGETED THERAPY | 2021年 / 6卷 / 01期

关键词：

SARS-COV-2; CELLS;

D O I：

10.1038/s41392-021-00861-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Messenger RNA (mRNA) vaccine technology has shown its power in preventing the ongoing COVID-19 pandemic. Two mRNA vaccines targeting the full-length S protein of SARS-CoV-2 have been authorized for emergency use. Recently, we have developed a lipid nanoparticle-encapsulated mRNA (mRNA-LNP) encoding the receptor-binding domain (RBD) of SARS-CoV-2 (termed ARCoV), which confers complete protection in mouse model. Herein, we further characterized the protection efficacy of ARCoV in nonhuman primates and the long-term stability under normal refrigerator temperature. Intramuscular immunization of two doses of ARCoV elicited robust neutralizing antibodies as well as cellular response against SARS-CoV-2 in cynomolgus macaques. More importantly, ARCoV vaccination in macaques significantly protected animals from acute lung lesions caused by SARS-CoV-2, and viral replication in lungs and secretion in nasal swabs were completely cleared in all animals immunized with low or high doses of ARCoV. No evidence of antibody-dependent enhancement of infection was observed throughout the study. Finally, extensive stability assays showed that ARCoV can be stored at 2-8 degrees C for at least 6 months without decrease of immunogenicity. All these promising results strongly support the ongoing clinical trial.

引用

页数：9