Role of autophagy in lysophosphatidylcholine-induced apoptosis in mouse Leydig cells

被引：5

作者：

Zeng, Lin ^{[1
,2
]}

Ma, Bingchun ^{[1
]}

Yang, Si ^{[1
]}

Zhang, Meijuan ^{[1
]}

Wang, Jinglei ^{[1
]}

Liu, Mengling ^{[1
,3
]}

Chen, Jiaxiang ^{[1
,4
]}

机构：

[1] Nanchang Univ, Sch Basic Med Sci, Dept Physiol, 461 Bayi Rd, Nanchang 330006, Jiangxi, Peoples R China

[2] Nanchang Emergency Ctr, Nanchang, Jiangxi, Peoples R China

[3] Jiujiang Univ, Nursing Sch, Jiujiang, Peoples R China

[4] Jiangxi Prov Key Lab Reprod Physiol & Pathol, Nanchang, Jiangxi, Peoples R China

来源：

ENVIRONMENTAL TOXICOLOGY | 2022年 / 37卷 / 11期

基金：

中国国家自然科学基金;

关键词：

apoptosis; autophagy; Leydig cells; lysophosphatidylcholine; oxidative stress; SPERMATOGENESIS; INVOLVEMENT; INHIBITION; DISEASE; INJURY;

D O I：

10.1002/tox.23634

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Lysophosphatidylcholine (LPC), a major class of glycerophospholipids ubiquitously present in most tissues, plays a dominant role in many diseases, while it is still unknown about the potential mechanism of LPC affecting the testicular Leydig cells. In the present study, mouse TM3 Leydig cells in vitro were treated with LPC for 48 h. LPC was found to significantly induce apoptosis and oxidative stress of mouse TM3 Leydig cells; while inhibition of oxidative stress by N-acetyl-L-cysteine, an inhibitor of oxidative stress, could rescue the induction of apoptosis, indicating that LPC induced apoptosis of mouse TM3 Leydig cells via oxidative stress. Interestingly, LPC was showed to inhibit autophagy; however, induction of autophagy by rapamycin significantly alleviated the induction of apoptosis by LPC. Taken together, oxidative stress was involved in LPC-induced apoptosis of mouse TM3 Leydig cells, and autophagy might play a protective role in LPC-induced apoptosis.

引用

页码：2756 / 2763

页数：8

共 46 条

[1] Phospholipase A2 Biochemistry [J].