New monolithic enzymatic micro-reactor for the fast production and purification of oligogalacturonides

被引：30
作者：
Delattre, C. [1 ]
Michaud, P. [2 ]
Vijayalakshmi, M. A. [1 ]
机构：
[1] Univ Vellore, Vellore Inst Technol, Ctr BioSeparat Technol, Vellore 632014, Tamil Nadu, India
[2] Univ Blaise Pascal, Polytech Clermont Ferrand, Lab Genie Chim & Biochim, F-63174 Aubiere, France
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2008年 / 861卷 / 02期
关键词：
CIM-disk; enzymatic bioreactor; monolith chromatography; oligouronides; pectin lyase;
D O I：
10.1016/j.jchromb.2007.08.036
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Fast production and purification of alpha-(1,4)-oligogalacturonides was investigated using a new enzymatic reactor composed of a monolithic matrix. Pectin lyase from Aspergillus japonicus (Sigma) was immobilized on CIM-disk epoxy monolith. Studies were performed on free pectin lyase and immobilized pectin lyase to compare the optimum temperature, optimum pH, and thermal stability. It was determined that optimum temperature for free pectin lyase and immobilized pectin lyase on monolithic support is 30C, and optimum pH is 5. Monolithic CIM-disk chromatography is one of the fastest liquid chromatographic method used for separation and purification of biomolecules due to high mass transfer rate. In this context, online one step production and purification of oligogalacturonides was investigated associating CIM-disk pectin lyase and CIM-disk DEAE. This efficient enzymatic bioreactor production of uronic oligosaccharides from polygalacturonic acid (PGA) constitutes an original fast process to generate bioactive oligouronides. (c) 2007 Elsevier B.V. All rights reserved.
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收藏
页码：203 / 208
页数：6
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