Human umbilical cord mesenchymal stem cells derived extracellular vesicles regulate acquired immune response of lupus mouse in vitro

被引：15

作者：

Xie, Min ^{[1
]}

Li, Cuifang ^{[1
]}

She, Zhou ^{[1
]}

Wu, Feifeng ^{[1
]}

Mao, Jueyi ^{[1
]}

Hun, Marady ^{[1
]}

Luo, Senlin ^{[1
]}

Wan, Wuqing ^{[1
]}

Tian, Jidong ^{[1
]}

Wen, Chuan ^{[1
]}

机构：

[1] Cent South Univ, Xiangya Hosp 2, Div Hematol & Tumor, Childrens Med Ctr, Changsha, Hunan, Peoples R China

来源：

SCIENTIFIC REPORTS | 2022年 / 12卷 / 01期

基金：

中国国家自然科学基金;

关键词：

FOLLICULAR HELPER-CELL; AUTOIMMUNE-DISEASES; STROMAL CELLS; TRANSPLANTATION; INTERLEUKIN-10; ERYTHEMATOSUS; MRL/LPR; RECEPTOR; BLOOD; DIFFERENTIATION;

D O I：

10.1038/s41598-022-17331-8

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Systemic lupus erythematosus (SLE) is an autoimmune disease involving multiple systems. Immunopathology believes that abnormal T cell function and excessive production of autoantibodies by B cells are involved in multi-organ damage. Human umbilical cord mesenchymal stem cells (hUCMSCs) therapies have endowed with promise in SLE, while the function of MSC-derived extracellular vesicles (MSC-EVs) was still unclear. Extracellular vesicles (EVs) are subcellular components secreted by a paracellular mechanism and are essentially a group of nanoparticles. EVs play a vital role in cell-to-cell communication by acting as biological transporters. New evidence has shown beneficial effects of MSC-EVs on autoimmune diseases, such as their immunomodulatory properties. In this study, we investigated whether hUCMSCs derived extracellular vesicles (hUCMSC-EVs) could regulate abnormal immune responses of T cells or B cells in SLE. We isolated splenic mononuclear cells from MRL/lpr mice, a classical animal model of SLE. PBS (Phosphate-buffered saline), 2 x 10(5) hUCMSCs, 25 mu g/ml hUCMSC-EVs, 50 mu g/ml hUCMSC-EVs were co-cultured with 2 x 10(6) activated splenic mononuclear cells for 3 days in vitro, respectively. The proportions of CD4(+) T cell subsets, B cells and the concentrations of cytokines were detected. Both hUCMSCs and hUCMSC-EVs inhibited CD4(+) T cells, increased the production of T helper (Th)17 cells, promoted the production of interleukin (IL)-17 and transforming growth factor beta1 (TGF-beta 1) (P < 0.05), although they had no significant effects on Th1, Th2, T follicular helper (Tfh), regulatory T (Treg) cells and IL-10 (P > 0.05); only hUCMSCs inhibited CD19(+) B cells, promoted the production of interferon-gamma (IFN-gamma) and IL-4 (P < 0.05). hUCMSCs exert immunoregulatory effects on SLE at least partially through hUCMSC-EVs in vitro, therefore, hUCMSC-EVs play novel and potential regulator roles in SLE.

引用

页数：9

共 52 条

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