Use of Antigen Combinations to Address Complex Leishmania-Seropositivity Patterns in Dogs Living in Canine Leishmaniosis Endemic Regions of Portugal

被引:5
作者
Lima, Carla Silva [1 ,2 ,3 ]
Esteves, Sofia [1 ,2 ,3 ]
Costa, Ines [1 ,2 ,3 ]
Brancal, Hugo [4 ]
Lima, Clara [1 ,2 ,3 ]
Amorim, Celia [1 ,2 ,9 ]
Cardoso, Luis [5 ,6 ,7 ,8 ]
Santarem, Nuno [1 ,2 ,3 ]
Cordeiro-da-Silva, Anabela [1 ,2 ,3 ]
机构
[1] Univ Porto, Inst Invest & Inovacao Saude, P-4200135 Porto, Portugal
[2] Univ Porto, Inst Biol Mol & Celular, P-4200135 Porto, Portugal
[3] Univ Porto, Dept Ciencias Biol, Serv Microbiol, Fac Farm, P-4050313 Porto, Portugal
[4] Clin Vet Covilha, P-6200293 Covilha, Portugal
[5] Univ Beira Interior, Fac Ciencias Saude, P-6200506 Covilha, Portugal
[6] Univ Tras Os Montes & Alto Douro UTAD, Dept Ciencias Vet, Escola Ciencias Agr & Vet, P-5000801 Vila Real, Portugal
[7] UTAD, Ctr Ciencia Anim & Vet CECAV, P-5001801 Vila Real, Portugal
[8] Univ Tras Os Montes & Alto Douro UTAD, Lab Associado Ciencia Anim & Vet AL4Anim, P-5000801 Vila Real, Portugal
[9] Univ Porto, Dept Ciencias Quim, Fac Farm, P-4200135 Porto, Portugal
关键词
Canine leishmaniosis; clinical infection; DAT; ELISA; Leishmania antigens; rK39; serological tests; subclinical infection; DIRECT AGGLUTINATION-TEST; VISCERAL LEISHMANIASIS; SEROLOGIC DIAGNOSIS; INFANTUM; INFECTION; SERODIAGNOSIS; ASSAYS; TESTS; ELISA; PCR;
D O I
10.3390/microorganisms10102018
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Canine leishmaniosis (CanL) is a vector-borne disease caused by Leishmania infantum. Infection in dogs can result in a disease with non-specific clinical signs or in a subclinical condition. Infection diagnosis is crucial to guide public health measures considering the zoonotic potential of L. infantum. Serological approaches to detect infection with a reduced antigen panel potentially limit the quality of the information obtained. To evaluate the impact of using distinct antigens in a serological survey, a cohort with 390 dogs from endemic regions in Portugal was subjected to a serological evaluation using ELISA and DAT. Using ELISA, six Leishmania-specific antigens in conjunction with a non-related antigen, Escherichia coli soluble antigens, were evaluated. The global seroprevalence was 10.5% for DAT and 15.4 to 23.1% for ELISA, depending on the antigen for the latter. Still, only 8.2% of the animals were seropositive to all Leishmania-specific antigens. Importantly, a further 31.0% presented antigen-dependent seropositivity. Considering this observation, a serological score system was proposed and validated to address the complex serology results. With this system, the overall dog seropositivity was 26.9%. This work highlights the limitations of single-antigen serological surveys and presents an approach that might contribute to the establishment of CanL-specific serological profiles.
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页数:15
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