Enhancing Nanoparticle-Based Visible Detection by Controlling the Extent of Aggregation

被引：41

作者：

Lim, Seokwon ^{[1
,2
,3
]}

Koo, Ok Kyung ^{[4
]}

You, Young Sang ^{[2
]}

Lee, Yeong Eun ^{[2
]}

Kim, Min-Sik ^{[5
]}

Chang, Pahn-Shick ^{[1
,2
]}

Kang, Dong Hyun ^{[1
,2
]}

Yu, Jae-Hyuk ^{[6
]}

Choi, Young Jin ^{[1
,2
]}

Gunasekaran, Sundaram ^{[3
]}

机构：

[1] Seoul Natl Univ, Ctr Agr Biomat, Seoul 151742, South Korea

[2] Seoul Natl Univ, Dept Agr Biotechnol, Seoul 151742, South Korea

[3] Univ Wisconsin, Dept Biol Syst Engn, Madison, WI 53706 USA

[4] Univ Arkansas, Dept Food Sci, Ctr Food Safety, Fayetteville, AR 72704 USA

[5] Univ Wisconsin, Sch Pharm, Div Pharmaceut Sci, Madison, WI 53705 USA

[6] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA

来源：

SCIENTIFIC REPORTS | 2012年 / 2卷

关键词：

GOLD NANOPARTICLES; COLORIMETRIC DETECTION; ESCHERICHIA-COLI; BINDING; DESIGN; SENSOR;

D O I：

10.1038/srep00456

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Visible indication based on the aggregation of colloidal nanoparticles (NPs) is highly advantageous for rapid on-site detection of biological entities, which even untrained persons can perform without specialized instrumentation. However, since the extent of aggregation should exceed a certain minimum threshold to produce visible change, further applications of this conventional method have been hampered by insufficient sensitivity or certain limiting characteristics of the target. Here we report a signal amplification strategy to enhance visible detection by introducing switchable linkers (SLs), which are designed to lose their function to bridge NPs in the presence of target and control the extent of aggregation. By precisely designing the system, considering the quantitative relationship between the functionalized NPs and SLs, highly sensitive and quantitative visible detection is possible. We confirmed the ultrahigh sensitivity of this method by detecting the presence of 20 fM of streptavidin and fewer than 100 CFU/mL of Escherichia coli.

引用

页数：6

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