Electrospray mass spectra from protein electroeluted from sodium dodecylsulfate polyacrylamide gel electrophoresis gels

被引：29

作者：

Fridriksson, EK ^{[1
]}

Baird, B ^{[1
]}

McLafferty, FW ^{[1
]}

机构：

[1] Cornell Univ, Baker Lab Chem, Dept Chem & Biol Chem, Ithaca, NY 14853 USA

来源：

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 1999年 / 10卷 / 05期

关键词：

D O I：

10.1016/S1044-0305(99)00012-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Electrospray ionization/tandem mass spectrometry of proteins separated on sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) gels is severely limited by the requirement that the protein be completely separated from the SDS. As shown here, the gaseous noncovalent SDS adducts of protonated proteins thus formed can be dissociated by infrared irradiation. ESI spectra from myoglobin in SDS-containing solutions show molecular ions adducted with up to 15 dodecyl sulfates, but ir irradiation of these ions causes complete dissociation to expel the SDS. (C) 1999 American Society for Mass Spectrometry.

引用

页码：453 / 455

页数：3

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