Effects of Allium sativum Stem Extract on Growth and Migration in Melanoma Cells through Inhibition of VEGF, MMP-2, and MMP-9 Genes Expression

被引:9
|
作者
Gam, Da-Hye [1 ]
Park, Jae-Hyun [1 ]
Kim, Jun-Hee [1 ]
Beak, Dong-Ho [1 ]
Kim, Jin-Woo [1 ,2 ]
机构
[1] Sun Moon Univ, Dept Food Sci, Nat Sci 118,70 Sunmoon Ro 221, Asan 336708, South Korea
[2] FlexPro Biotechnol, Nat Sci 128,70 Sunmoon Ro 221, Asan 336708, South Korea
来源
MOLECULES | 2022年 / 27卷 / 01期
关键词
Allium sativum stem; anticancer; antimetastasis; VEGF; MMP-2; MMP-9; gallic acid; OVARIAN-CARCINOMA; METASTASIS; FUCOIDAN; ASCITES;
D O I
10.3390/molecules27010021
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The present study investigated the effects of Allium sativum stem extract (ASE) on B16-F0 cell growth and metastasis. Evaluation of the effects of ASE on B16-F0 cells' viability and migration showed that 0.5 mg/mL ASE inhibited B16-F0 cells' growth by 30.2% and migration by 38.5%, which indicates that the ASE has anticancer and antimetastatic effects on B16-F0 cells. To study the anticancer and antimetastatic mechanism, mRNA levels of vascular endothelial growth factor (VEGF), matrix metalloproteinases-2 (MMP-2), and matrix metalloproteinases-9 (MMP-9) expressions were evaluated with reverse transcription polymerase chain reaction, and 0.25 and 0.5 mg/mL ASE was found to exert significant inhibition on mRNA expressions of VEGF, MMP-2, and MMP-9 in B16-F0 cells. Thus, ASE reduce extracellular matrix degradation through inhibitions of expression of MMP-2 and MMP-9, and also showed an angiogenesis inhibitory effect through reduction of VEGF expression. High-performance liquid chromatography analysis showed that among various polyphenols, gallic acid (2.1 mg/g) was a major compound of ASE. Overall, our results demonstrated that ASE inhibited the growth and migration of B16-F0 cells through downregulation of the VEGF, MMP-2, and MMP-9 genes expression, which indicates ASE could be applied for the prevention and treatment of melanoma.
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页数:12
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