A high fidelity CRISPR/Cas12a based lateral flow biosensor for the detection of HPV16 and HPV18

被引：90

作者：

Mukama, Omar ^{[1
,3
,7
]}

Yuan, Ting ^{[4
,5
]}

He, Zhixu ^{[4
,6
]}

Li, Zhiyuan ^{[1
]}

Habimana, Jean de Dieu ^{[1
,3
]}

Hussain, Muzammal ^{[1
,3
]}

Li, Wei ^{[5
]}

Yi, Zhijian ^{[2
]}

Liang, Qiongxin ^{[2
]}

Zeng, Lingwen ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, South China Inst Stem Cell Biol & Regenerat Med, Guangzhou Inst Biomed & Hlth, Key Lab Regenerat Biol, Guangzhou 510530, Peoples R China

[2] Foshan Univ, Sch Food Sci & Engn, Foshan 528231, Peoples R China

[3] Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China

[4] Guizhou Med Univ, Dept Immunol, Guizhou Prov Key Lab Regenerat Med, Guiyang, Peoples R China

[5] Zunyi Med Univ, Dept Blood Transfus, Peoples Hosp Zunyi 1, Affiliated Hosp 3, Zunyi, Guizhou, Peoples R China

[6] Zunyi Med Univ, Dept Pediat, Affiliated Hosp, Zunyi, Guizhou, Peoples R China

[7] Univ Rwanda, Coll Sci & Technol, Dept Appl Biol, Ave Armee,POB 3900, Kigali, Rwanda

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2020年 / 316卷

基金：

中国国家自然科学基金;

关键词：

CRISPR/Cas12a; Loop-mediated isothermal amplification; Lateral flow biosensor; Nucleic acid detection; Human papilloma virus; NUCLEIC-ACID DETECTION; HUMAN-PAPILLOMAVIRUS; SPECIFICITIES; AMPLIFICATION; CANCER;

D O I：

10.1016/j.snb.2020.128119

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Abnormal growth of uterine cervix cells may eventually lead to cervical cancer, which is mainly associated with the two most prevalent types of Human Papilloma Virus (HPV), namely HPV16 and HPV18. Cervical cancer can be prevented or treated owing to its slow progression. Therefore, its early, fast, and sensitive diagnosis is crucial. Herein, we developed an ultrasensitive approach for HPV16 and HPV18 detection based on a combination of loop-mediated isothermal amplification (LAMP), CRISPR-Cas12a trans-cleavage propensity and lateral flow biosensor (LFB), collectively termed CIALFB (CRISPR/Cas-Isothermal Amplification based LFB). CIALFB is characterized by Cas12a-mediated trans-cleavage of the reporter ssDNA upon target recognition, which results in undetectable LFB test line signal. This method was highly sensitive to detect 3.1 attomoles (similar to 1.8 copies) of the target, and reliably specific to detect both virus types from clinical samples with various HPV strains. Our system possesses the potential to detect other infectious diseases without tedious DNA extraction and handling, and expensive apparatuses.

引用

页数：8

共 30 条

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