Phenolic Constituents of Eucalyptus camaldulensis Dehnh, with Potential Antioxidant and Cytotoxic Activities

被引：0

作者：

Singab, Abdel-Nasser ^{[1
]}

Ayoub, Nahla ^{[1
]}

Al-Sayed, Eman ^{[1
,2
]}

Martiskainen, Olli ^{[2
]}

Sinkkonen, Jari ^{[2
]}

Pihlaja, Kalevi ^{[2
]}

机构：

[1] Ain Shams Univ, Fac Pharm, Dept Pharmacognosy, Cairo 11566, Egypt

[2] Univ Turku, Dept Chem, Lab Organ Chem & Chem Biol, FI-20014 Turku, Finland

来源：

RECORDS OF NATURAL PRODUCTS | 2011年 / 5卷 / 04期

关键词：

Myrtaceae; Eucalyptus camaldulensis; DPPH; 2-deoxy-2-ribose; HPLC-PDA-ESI/MS/MS; cytotoxicity; CANCER CHEMOPREVENTION; ESSENTIAL OILS; VAR; OBTUSA; ACID; GLYCOSIDES; PRODUCTS; LEAVES;

D O I：

暂无

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A liquid chromatography-diode array detection-electrospray ionization mass spectrometric (HPLC-PDA-ESI/MS/MS) method was used for separation and characterization of the phytoconstituents of the aqueous acetone leaf extract of Eucalyptus camaldulensis Dehnh (Myrtaceae). The employed method was optimized for separation, identification and quantification of fifty six compounds including ellagitannins, flavonoids, phloroglucinol derivatives and galloyl esters. The antioxidant effect was determined in vitro using 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical and super oxide anion radical scavenging assays. The cytotoxicity of the aqueous acetone extract was evaluated on MCF-7, Hep-2, HepG-2, HeLa, HCT-116 and Caco-2 cell lines. The results indicated that most of the fractions exhibited strong antioxidant activity. The aqueous acetone extract reduced the viability of all cell lines in a dose-dependent manner, and was more active on MCF-7 and HCT-116 cell lines.

引用

页码：271 / 280

页数：10

共 23 条

[1] FLAVONOID GLYCOSIDES AND THE CHEMOSYSTEMATICS OF EUCALYPTUS-CAMALDULENSIS [J].

ABDALLA, MF ;

ELNEGOUMY, SI ;

ELLAKANY, MH ;

SALEH, NAM .

PHYTOCHEMISTRY, 1980, 19 (12) :2629-2632

[2] Constituents and their antioxidative effects in eucalyptus leaf extract used as a natural food additive [J].

Amakura, Y ;

Umino, Y ;

Tsuji, S ;

Ito, H ;

Hatano, T ;

Yoshida, T ;

Tonogai, Y .

FOOD CHEMISTRY, 2002, 77 (01) :47-56

[3] Structure and spasmolytic activity of eucalyptanoic acid from Eucalyptus camaldulensis var. obtusa and synthesis of its active derivative from oleanolic acid [J].

Begum, S ;

Sultana, I ;

Siddiqui, BS ;

Shaheen, F ;

Gilani, AH .

JOURNAL OF NATURAL PRODUCTS, 2002, 65 (12) :1939-1941

[4] Spasmolytic constituents from Eucalyptus camaldulensis var. obtusa leaves [J].

Begum, S ;

Farhat ;

Sultana, I ;

Siddiqui, BS ;

Shaheen, F ;

Gilani, AH .

JOURNAL OF NATURAL PRODUCTS, 2000, 63 (09) :1265-1268

[5]

Dewanto V, 2002, J AGR FOOD CHEM, V50, P3010, DOI [10.1021/jf0115589, 10.1021/jf0255937]

[6] The Egyptian Eucalyptus camaldulensis var. brevirostris:: chemical compositions of the fruit volatile oil and antioxidant activity [J].

El-Ghorab, AH ;

Fadel, HM ;

El-Massry, KF .

FLAVOUR AND FRAGRANCE JOURNAL, 2002, 17 (04) :306-312

[7] Eucalyptus camaldulensis:: volatiles from immature flowers and high production of 1,8-cineole and β-pinene by in vitro cultures [J].

Giamakis, A ;

Kretsi, O ;

Chinou, I ;

Spyropoulos, CG .

PHYTOCHEMISTRY, 2001, 58 (02) :351-355

[8] Antioxidant activity of L-adrenaline: A structure-activity insight [J].

Guelcin, Ilhami .

CHEMICO-BIOLOGICAL INTERACTIONS, 2009, 179 (2-3) :71-80

[9] FORMATION OF A THIOBARBITURIC-ACID-REACTIVE SUBSTANCE FROM DEOXYRIBOSE IN THE PRESENCE OF IRON SALTS - THE ROLE OF SUPEROXIDE AND HYDROXYL RADICALS [J].

HALLIWELL, B ;

GUTTERIDGE, JMC .

FEBS LETTERS, 1981, 128 (02) :347-352

[10] Bioactive monoterpene glycosides conjugated with gallic acid from the leaves of Eucalyptus globulus [J].

Hasegawa, Tatsuya ;

Takano, Fumihide ;

Takata, Takanobu ;

Niiyama, Masato ;

Ohta, Tomillisa .

PHYTOCHEMISTRY, 2008, 69 (03) :747-753

← 1 2 3 →