Self-nanoemulsifying drug delivery systems as novel approach for pDNA drug delivery

被引：62
作者：
Hauptstein, Sabine [1 ]
Pruefert, Felix [1 ]
Bernkop-Schnuerch, Andreas [1 ]
机构：
[1] Univ Innsbruck, Ctr Chem & Biomed, Inst Pharm, Dept Pharmaceut Technol, A-6020 Innsbruck, Austria
来源：
INTERNATIONAL JOURNAL OF PHARMACEUTICS | 2015年 / 487卷 / 1-2期
关键词：
SNEDDS; Nonviral vector; Hydrophobic ion pairing; Gene delivery; VIVO EVALUATION; GENE DELIVERY; IN-VITRO; CELL; PROTEIN; SMEDDS;
D O I：
10.1016/j.ijpharm.2015.03.064
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
It was the aim of this study to investigate a novel strategy for oral gene delivery utilizing a self-nanoemulsifying drug delivery system (SNEDDS). After hydrophobic ion pairing a plasmid was incorporated into SNEDDS. The mean droplet size of resulting nanoemulsions was determined to be between 45.8 and 47.5 nm. A concentration dependent cytotoxicity of the formulations was found on HEK-293 cells via MTT assay. Degradation studies via DNase I showed that incorporation into SNEDDS led to significantly, up to 8-fold prolonged resistant time against enzymatic digestion compared to naked pDNA and pDNA-lipid complexes. Transfection studies carried out revealed a significantly improved transfection compared to naked pDNA. Further, no decrease in transfection efficiency compared to transfection using Lipofectin (R) transfection reagent was observed. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码：25 / 31
页数：7
相关论文
共 18 条
[1]   Drug delivery strategies for poorly water-soluble drugs [J].
Fahr, Alfred ;
Liu, Xiangli .
EXPERT OPINION ON DRUG DELIVERY, 2007, 4 (04) :403-416
[2]  
FELGNER JH, 1994, J BIOL CHEM, V269, P2550
[3]   Development and Evaluation of a Novel Mucus Diffusion Test System Approved by Self-Nanoemulsifying Drug Delivery Systems [J].
Friedl, Heike ;
Duennhaupt, Sarah ;
Hintzen, Fabian ;
Waldner, Claudia ;
Parikh, Shruti ;
Pearson, Jeffrey P. ;
Wilcox, Matthew D. ;
Bernkop-Schnuerch, Andreas .
JOURNAL OF PHARMACEUTICAL SCIENCES, 2013, 102 (12) :4406-4413
[4]  
Hagiwara Kenji, 2011, Curr Drug Discov Technol, V8, P329
[5]   Thiomers and thiomer-based nanoparticles in protein and DNA drug delivery [J].
Hauptstein, Sabine ;
Bernkop-Schnuerch, Andreas .
EXPERT OPINION ON DRUG DELIVERY, 2012, 9 (09) :1069-1081
[6]   In vitro and ex vivo evaluation of an intestinal permeation enhancing self-microemulsifying drug delivery system (SMEDDS) [J].
Hintzen, F. ;
Laffleur, F. ;
Sarti, F. ;
Mueller, C. ;
Bernkop-Schnuerch, A. .
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY, 2013, 23 (03) :261-267
[7]   In vivo evaluation of an oral self-microemulsifying drug delivery system (SMEDDS) for leuprorelin [J].
Hintzen, Fabian ;
Perera, Glen ;
Hauptstein, Sabine ;
Mueller, Christiane ;
Laffleur, Flavia ;
Bernkop-Schnuerch, Andreas .
INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2014, 472 (1-2) :20-26
[8]   Development of self-microemulsifying drug delivery systems (SMEDDS) for oral bioavailability enhancement of simvastatin in beagle dogs [J].
Kang, BK ;
Lee, JS ;
Chon, SK ;
Jeong, SY ;
Yuk, SH ;
Khang, G ;
Lee, HB ;
Cho, SH .
INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2004, 274 (1-2) :65-73
[9]   Triton X-100 concentration effects on membrane permeability of a single HeLa cell by scanning electrochemical microscopy (SECM) [J].
Koley, Dipankar ;
Bard, Allen J. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (39) :16783-16787
[10]   Hydrophobic ion pairing: Altering the solubility properties of biomolecules [J].
Meyer, JD ;
Manning, MC .
PHARMACEUTICAL RESEARCH, 1998, 15 (02) :188-193
12