Identification of Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa in Blood Cultures: a Multicenter Performance Evaluation of a Three-Color Peptide Nucleic Acid Fluorescence In Situ Hybridization Assay

被引：18

作者：

Della-Latta, Phyllis ^{[2
]}

Salimnia, Hossein ^{[3
,4
]}

Painter, Theresa ^{[3
]}

Wu, Fann ^{[2
]}

Procop, Gary W. ^{[5
]}

Wilson, Deborah A. ^{[5
]}

Gillespie, Wendy ^{[6
]}

Mender, Alayna ^{[1
]}

Crystal, Benjamin S. ^{[1
]}

机构：

[1] AdvanDx Inc, Woburn, MA 01801 USA

[2] Columbia Univ, Med Ctr, NewYork Presbyterian Hosp, New York, NY USA

[3] Detroit Med Ctr, Detroit, MI USA

[4] Wayne State Univ, Sch Med, Detroit, MI USA

[5] Cleveland Clin, Cleveland, OH 44106 USA

[6] Lahey Clin Fdn, Burlington, MA USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2011年 / 49卷 / 06期

关键词：

GRAM-NEGATIVE BACILLI; ANTIMICROBIAL THERAPY; CANDIDA-ALBICANS; RISK-FACTORS; MORTALITY; IMPACT;

D O I：

10.1128/JCM.02250-10

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A multicenter evaluation was undertaken to evaluate the performance of a new three-color peptide nucleic acid fluorescence in situ hybridization assay that identifies isolates directly from blood cultures positive for Gram-negative bacilli (GNB). The assay correctly identified 100% (186/186) of the Escherichia coli isolates, 99.1% (109/110) of the Klebsiella pneumoniae isolates, and 95.8% (46/48) of the Pseudomonas aeruginosa isolates in this study. Negative assay results were correctly obtained for 162 of 165 other GNB (specificity, 98.2%).

引用

页码：2259 / 2261

页数：3

共 10 条

[1] Cost savings with implementation of PNA FISH testing for identification of Candida albicans in blood cultures [J].

Alexander, BD ;

Ashley, ED ;

Reller, LB ;

Reed, SD .

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2006, 54 (04) :277-282

[2] Peptide nucleic acid fluorescence in situ hybridization-based identification of Candida albicans and its impact on mortality and antifungal therapy costs [J].

Forrest, G. N. ;

Mankes, K. ;

Jabra-Rizk, M. A. ;

Weekes, E. ;

Johnson, J. K. ;

Lincalis, D. P. ;

Venezia, R. A. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (09) :3381-3383

[3] Impact of rapid in situ hybridization testing on coagulase-negative staphylococci positive blood cultures [J].

Forrest, Graerne N. ;

Mehta, Sanjay ;

Weekes, Elizabeth ;

Lincalis, Durry P. ;

Johnson, Jennifer K. ;

Venezia, Richard A. .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2006, 58 (01) :154-158

[4] Overview of nosocomial infections caused by gram-negative bacilli [J].

Gaynes, R ;

Edwards, JR .

CLINICAL INFECTIOUS DISEASES, 2005, 41 (06) :848-854

[5]

HANSEN DS, 2010, 20 ESCMID VIENN AUST

[6] Pseudomonas aeruginosa bacteremia:: Risk factors for mortality and influence of delayed receipt of effective antimicrobial therapy on clinical outcome [J].

Kang, CI ;

Kim, SH ;

Kim, HB ;

Park, SW ;

Choe, YJ ;

Oh, MD ;

Kim, EC ;

Choe, KW .

CLINICAL INFECTIOUS DISEASES, 2003, 37 (06) :745-751

[7] Bloodstream infections caused by antibiotic-resistant gram-negative bacilli: Risk factors for mortality and impact of inappropriate initial antimicrobial therapy on outcome [J].

Kang, CI ;

Kim, SH ;

Park, WB ;

Lee, KD ;

Kim, HB ;

Kim, EC ;

Oh, MD ;

Choe, KW .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2005, 49 (02) :760-766

[8] Prevalence and antimicrobial susceptibilities of bacteria isolated from blood cultures of hospitalized patients in the United States in 2002 [J].

Karlowsky J.A. ;

Jones M.E. ;

Draghi D.C. ;

Thornsberry C. ;

Sahm D.F. ;

Volturo G.A. .

Annals of Clinical Microbiology and Antimicrobials, 3 (1)

[9] Pseudomonas aeruginosa bloodstream infection:: Importance of appropriate initial antimicrobial treatment [J].

Micek, ST ;

Lloyd, AE ;

Ritchie, DJ ;

Reichley, RM ;

Fraser, VJ ;

Kollef, MH .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2005, 49 (04) :1306-1311

[10] Multicenter Evaluation of a New Shortened Peptide Nucleic Acid Fluorescence In Situ Hybridization Procedure for Species Identification of Select Gram-Negative Bacilli from Blood Cultures [J].

Morgan, Margie ;

Marlowe, Elizabeth ;

Della-Latta, Phyllis ;

Salimnia, Hossein ;

Novak-Weekley, Susan ;

Wu, Fann ;

Crystal, Benjamin S. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2010, 48 (06) :2268-2270

← 1 →