PCR system for the correct differentiation of the main bacterial species of the Klebsiella pneumoniae complex

被引：6

作者：

Humberto, Barrios-Camacho ^{[1
]}

Jesus, Silva-Sanchez ^{[1
]}

Elena, Cercas-Ayala ^{[1
]}

Luis, Lozano-Aguirre ^{[2
]}

Josefina, Duran-Bedolla ^{[1
]}

Alejandro, Aguilar-Vera ^{[3
]}

Elvira, Garza-Gonzalez ^{[4
]}

Paola, Bocanegra-Ibarias ^{[4
]}

Rayo, Morfin-Otero ^{[5
]}

Rigoberto, Hernandez-Castro ^{[6
]}

Ulises, Garza-Ramos ^{[1
]}

机构：

[1] Inst Nacl Salud Publ INSP, Ctr Invest Enfermedades Infecciosas CISEI, Lab Resistencia Bacteriana, Av Univ 655, Cuernavaca 62100, Morelos, Mexico

[2] Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Programa Genom Evolut, Cuernavaca, Morelos, Mexico

[3] Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Programa Genom Func Procariotes, Cuernavaca, Morelos, Mexico

[4] Univ Autonoma Nuevo Leon, Hosp Univ Dr Jose Eleuterio Gonzalez, Monterrey, Nuevo Leon, Mexico

[5] Univ Guadalajara, Hosp Civil Guadalajara Fray Antonio Alcalde, Guadalajara, Jalisco, Mexico

[6] Hosp Gen Dr Manuel Gea Gonzalez, Dept Agentes Patogenos, Mexico City, DF, Mexico

来源：

ARCHIVES OF MICROBIOLOGY | 2022年 / 204卷 / 01期

关键词：

Misclassification; Unique genes; Proteome; Genomics; Klebsiella; Multiplex-PCR; Phylogenetic analysis; VARIICOLA; QUASIPNEUMONIAE;

D O I：

10.1007/s00203-021-02668-x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Accurate recognition of the closely related species Klebsiella pneumoniae, Klebsiella quasipneumoniae and Klebsiella variicola by phenotypic, biochemical and automated tests is notoriously unreliable in hospitals' diagnostic laboratories. A comparative genomics approach was conducted for the correct differentiation of the main bacterial species in the K. pneumoniae complex. Analysis of the deduced proteomes of 87 unique genomes of the Klebsiella in public databases, was used for the identification of unique protein family members. This allowed the design of a multiplex-PCR assay for the correct differentiation of these three species from different origins. This system allowed us to determine the prevalence of K. pneumoniae, K. quasipneumoniae and K. variicola among a collection of 552 clinical isolates. Of these, 87.3% (482/552) isolates corresponded to K. pneumoniae, 6.7% (33/552) to K. quasipneumoniae and 5.9% (33/552) to K. variicola. The multiplex-PCR results showed a 100% accuracy for the correct identification of the three species evaluated, which was validated with rpoB phylogenetic sequence analysis.

引用

页数：5

共 24 条

[1] Draft Genome Sequence of the First Hypermucoviscous Klebsiella quasipneumoniae subsp. quasipneumoniae Isolate from a Bloodstream Infection [J].