Effect of 5-aminolevulinic acid-based photodynamic therapy via reactive oxygen species in human cholangiocarcinoma cells

被引：49

作者：

Kim, Cy Hyun ^{[1
,2
]}

Chung, Chung-Wook ^{[1
]}

Choi, Kyung Ha ^{[1
]}

Yoo, Jin-Ju ^{[1
]}

Kim, Do Hyung ^{[1
,2
]}

Jeong, Young-Il ^{[1
]}

Kang, Dae Hwan ^{[1
,2
]}

机构：

[1] Pusan Natl Univ, Yangsan Hosp, Natl Res & Dev Ctr Hepatobiliary Canc, Res Inst Convergence Biomed Sci & Technol, Yangsan, South Korea

[2] Pusan Natl Univ, Sch Med, Yangsan, South Korea

来源：

INTERNATIONAL JOURNAL OF NANOMEDICINE | 2011年 / 6卷

关键词：

ALA-based photodynamic therapy; HuCC-T1; protoporphyrin IX; ROS; PROTOPORPHYRIN-IX ACCUMULATION; BILIARY EPITHELIAL-CELLS; CARCINOMA; APOPTOSIS; CANCER; LINES; PROLIFERATION; FLUORESCENCE; MICROSCOPY; MELANOMA;

D O I：

10.2147/IJN.S21395

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Cancer cells have been reported to exhibit an enhanced capacity for protoporphyrin IX (PpIX) synthesis facilitated by the administration of 5-aminolevulinic acid (ALA). We investigated the effect of ALA-based photodynamic therapy (PDT) on human cholangiocarcinoma cells (HuCC-T1). Since protoporphyrin IX (PpIX), a metabolite of ALA, can produce reactive oxygen species (ROS) under irradiation and then induce phototoxicity, ALA-based PDT is a promising candidate for the treatment of cholangiocarcinoma. When various concentrations of ALA (0.05-2 mM) were used to treat HuCC-T1 cells for 6 or 24 hours, the intracellular PpIX level increased according to the ALA concentration and treatment time. Furthermore, an increased amount of PpIX in HuCC-T1 cells induced increased production of ROS by irradiation, resulting in increased phototoxicity.

引用

页码：1357 / 1363

页数：7

共 32 条

[1] CD40 activation-induced, Fas-dependent apoptosis and NF-κB/AP-1 signaling in human intrahepatic biliary epithelial cells [J].