Molecular cloning and expression of the rat EAAT4 glutamate transporter subtype

被引:50
|
作者
Lin, CLG
Tzingounis, AV
Jin, L
Furuta, A
Kavanaugh, MP
Rothstein, JD
机构
[1] Johns Hopkins Univ, Dept Neurol, Baltimore, MD 21287 USA
[2] Oregon Hlth Sci Univ, Vollum Inst, Portland, OR 97201 USA
来源
MOLECULAR BRAIN RESEARCH | 1998年 / 63卷 / 01期
关键词
glutamate transporter; cerebellum; Purkinje cell; cDNA; ligand-gated chloride channel;
D O I
10.1016/S0169-328X(98)00256-3
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Glutamate transport is a primary mechanism for the synaptic inactivation of glutamate. Excitatory amino acid transporter 4 (EAAT4) is a novel glutamate transporter with properties of a ligand-gated chloride channel that was recently cloned from human brain. Here we report the cloning of rat EAAT4 (rEAAT4) cDNA from rat cerebellum. The nucleotide sequence of rEAAT4 was 88% identical to the human sequence, and the predicted peptide was 89% identical to the human protein. The transport activity encoded by rEAAT4 has high affinity for L-glutamate. In Xenopus laevis oocytes expressing rEAAT4, L-glutamate and other transporter substrates elicited a current predominantly carried by chloride ions. Like human EAAT4, the rEAAT4 mRNA was largely restricted to cerebellar Purkinje cells; the rEAAT4 protein was localized to Purkinje cell somas and dendrites. (C) 1998 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:174 / 179
页数:6
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