Genome sequence of a multidrug-resistant Campylobacter coli strain isolated from a newborn with severe diarrhea in Lebanon

被引:4
|
作者
Halimeh, Fatima Bachir [1 ,2 ]
Rafei, Rayane [1 ]
Diene, Seydina M. [2 ]
Osman, Marwan [1 ,3 ]
Kassem, Issmat I. [4 ,5 ]
Akoum, Randa Jamal [6 ]
Moudani, Walid [1 ]
Hamze, Monzer [1 ]
Rolain, Jean-Marc [2 ]
机构
[1] Lebanese Univ, Fac Publ Hlth, Doctoral Sch Sci & Technol, Lab Microbiol Sante & Environm LMSE, Tripoli, Lebanon
[2] Aix Marseille Univ, IHU Mediterranee Infect, AP HM, Fac Med & Pharm,IRD,MEPHI, 19-21 Blvd Jean Moulin, F-13385 Marseille, France
[3] Cornell Univ, Coll Vet Med, Dept Populat Med & Diagnost Sci, Ithaca, NY 14850 USA
[4] Univ Georgia, Ctr Food Safety, 1109 Expt St, Griffin, GA 30223 USA
[5] Univ Georgia, Dept Food Sci & Technol, 1109 Expt St, Griffin, GA 30223 USA
[6] Clin Jamal & Akoum, Pediatre, Tripoli, Lebanon
关键词
ANTIMICROBIAL RESISTANCE; RISK-FACTORS; VIRULENCE; PREVALENCE; POULTRY; JEJUNI; EPIDEMIOLOGY; MECHANISMS; EMERGENCE; INFECTION;
D O I
10.1007/s12223-021-00921-w
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A multidrug-resistant (MDR) Campylobacter coli (C. coli) strain was isolated from a 2-month-old newborn who suffered from severe diarrhea in Lebanon. Here, whole-genome sequencing (WGS) analysis was deployed to determine the genetic basis of antimicrobial resistance and virulence in the C. coli isolate and to identify its epidemiological background (sequence type). The identity of the isolate was confirmed using API (R) Campy, MALDI-TOF, and 16S rRNA gene sequencing analysis. The antimicrobial susceptibility phenotype was determined using the disk diffusion assay. Our analysis showed that resistance to macrolide and quinolone was potentially associated with the presence of multiple point mutations in antibiotic targets on the chromosomal DNA. Furthermore, tetracycline and aminoglycoside resistance were encoded by genes on a pTet plasmid. The bla(OXA-61), which is associated with beta-lactam resistance, was also detected in the C. coli genome. A set of 30 genes associated with the virulence in C. coli was detected using WGS analysis. MLST analysis classified the isolate as belonging to a new sequence type (ST-9588), a member of ST-828 complex which is mainly associated with humans and chickens. Taking together, this study provides the first WGS analysis of Campylobacter isolated from Lebanon. The detection of a variety of AMR and virulence determinants strongly emphasizes the need for studying the burden of Campylobacter in Lebanon and the Middle East and North Africa (MENA) region, where information on campylobacteriosis is scant.
引用
收藏
页码:319 / 328
页数:10
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